Description

Methyltetrazine-Amido-Peg5-Alkyne is a multifunctional heterobifunctional crosslinker designed for advanced bioconjugation and click chemistry applications. This compound integrates a highly reactive methyltetrazine group for rapid, bioorthogonal inverse electron-demand Diels-Alder (IEDDA) reactions with a terminal alkyne for copper-catalyzed azide-alkyne cycloaddition (CuAAC), connected by a flexible, hydrophilic PEG5 spacer. It is essential for researchers and developers in life sciences and material science who require precise, stable, and efficient linking of biomolecules, polymers, or surfaces. Its unique structure enables multi-step, orthogonal labeling strategies critical for drug discovery, diagnostics, and the development of novel biomaterials.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: For site-specific labeling of antibodies, proteins, and peptides with cytotoxic payloads, fluorophores, or other functional molecules.
• Bioorthogonal Labeling in Live Cells: Enables real-time imaging and tracking of biomolecules through fast, specific IEDDA reactions with trans-cyclooctene (TCO)-tagged probes.
• Surface Functionalization and Material Science: Used to modify nanoparticles, hydrogels, and other polymeric materials with bioactive ligands or for creating crosslinked networks.
• Proteomics and Chemical Biology Tool Development: Serves as a key reagent for constructing activity-based probes (ABPs) and affinity-based probes for target identification and validation.
• Diagnostic Assay Development: Facilitates the creation of sensitive immunoassays and biosensors through the stable attachment of detection molecules.
• Polymer Chemistry: Acts as a versatile monomer or crosslinking agent for synthesizing smart polymers with tailored biological functionalities.

Basic Information

Product Name	Methyltetrazine-Amido-Peg5-Alkyne
CAS No.	2322322-23-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Methyltetrazine-PEG5-Alkyne; 3-Methyl-1,2,4,5-tetrazine-6-carboxamido-PEG5-propargyl ether; TZ-PEG5-Alkyne; Alkyne-PEG5-Methyltetrazine; Methyl-Tetrazine-Pentakis(ethylene glycol)-Prop-2-yn-1-yl ether; Heterobifunctional PEG crosslinker, Methyltetrazine and Alkyne; Propargyl-PEG5-Methyltetrazine
EINECS	Contact for details

Quality Control

Every batch of Methyltetrazine-Amido-Peg5-Alkyne is manufactured and tested under strict quality management systems. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure high purity and consistent performance. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment. Our commitment to quality supports applications in regulated research and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	Orange to red viscous liquid or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.