Description

L-Aspartic Acid, n-[(9H-Fluoren-9-Ylmethoxy)Carbonyl]-n-Methyl-, 1-(1,1-Dimethylethyl) Ester is a sophisticated, N-methylated Fmoc-protected amino acid derivative designed for advanced peptide synthesis. This compound is critical for introducing a protected aspartic acid residue with a tertiary amide linkage, enabling the construction of complex peptides with enhanced stability and specific conformational properties. It is an essential building block for researchers and manufacturers in pharmaceutical development, proteomics, and bioconjugation, where precise control over peptide structure and function is paramount.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key protected amino acid for the automated, sequential assembly of peptide chains, particularly where N-methylation is required to modulate bioactivity and stability.
• Pharmaceutical Intermediate: Serves as a crucial precursor in the research and development of peptide-based therapeutics, including hormone analogs, enzyme inhibitors, and targeted drug candidates.
• Bioconjugation and Probe Development: Used to incorporate modified aspartic acid sites into proteins or peptides for labeling, imaging, or creating antibody-drug conjugates (ADCs).
• Chemical Biology Research: Enables the study of protein-protein interactions, enzyme mechanisms, and structure-activity relationships (SAR) by providing site-specific modifications.
• Process Development & Scale-Up: Supports the transition from laboratory-scale research to cGMP manufacturing for clinical trial material and commercial API production.

Basic Information

Item	Detail
Product Name	L-Aspartic Acid, n-[(9H-Fluoren-9-Ylmethoxy)Carbonyl]-n-Methyl-, 1-(1,1-Dimethylethyl) Ester
CAS No.	2271413-88-6
Molecular Formula	C27H31NO6
Molecular Weight	465.54 g/mol
Synonyms	Fmoc-N-Me-Asp(OtBu)-OH; Fmoc-N-methyl-L-aspartic acid 4-tert-butyl ester; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-N-methyl-L-aspartic acid 1-(1,1-dimethylethyl) ester; Nα-Fmoc-N-methyl-L-aspartic acid β-tert-butyl ester; Fmoc-Asp(OMe)-OtBu (common abbreviation); N-Fmoc-N-methylaspartic acid tert-butyl ester; (2S)-4-tert-Butoxy-2-[[(9H-fluoren-9-ylmethoxy)carbonyl](methyl)amino]-4-oxobutanoic acid
EINECS	Contact for details

Quality Control

Our L-Aspartic Acid, n-[(9H-Fluoren-9-Ylmethoxy)Carbonyl]-n-Methyl-, 1-(1,1-Dimethylethyl) Ester is produced under strict quality management systems. Every batch undergoes comprehensive analytical testing, including HPLC for purity and chiral integrity, to ensure it meets the exacting standards required for peptide synthesis. Certificates of Analysis (COA) with full chromatographic data are provided for traceability and regulatory compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and purity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Chiral Purity	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.