Description

n-Peg3-N'-(Azide-Peg3)-Cy5 is a bifunctional, near-infrared fluorescent labeling reagent designed for bioorthogonal conjugation. This compound matters for researchers and developers in life sciences as it combines a cyanine 5 (Cy5) fluorophore with two distinct poly(ethylene glycol) (PEG) linkers, one terminated with an azide group for copper-free click chemistry. Professionals in pharmaceutical R&D, bioconjugation, and diagnostic assay development need it for creating stable, fluorescently tagged probes, antibodies, and nanoparticles with enhanced solubility and reduced non-specific binding.

Application

• Bioconjugation and Probe Development: Site-specific labeling of proteins, antibodies, and peptides via strain-promoted azide-alkyne cycloaddition (SPAAC).
• In Vivo and In Vitro Imaging: Synthesis of near-infrared fluorescent probes for deep-tissue imaging and cellular tracking applications.
• Diagnostic Assay Reagents: Manufacturing components for fluorescence-based immunoassays, flow cytometry, and microscopy.
• Polymer and Nanoparticle Functionalization: Introducing fluorescent labels and click-chemistry handles onto PEGylated surfaces and drug delivery carriers.
• Chemical Biology Research: Tool compound for studying biomolecular interactions, localization, and dynamics using fluorescence.
• ADC (Antibody-Drug Conjugate) Payload-Linker Development: Exploration of novel linker-fluorophore constructs in targeted therapeutic platforms.

Basic Information

Product Name	n-Peg3-N'-(Azide-Peg3)-Cy5
CAS No.	2226235-96-5
Molecular Formula	C52H73N7O11
Molecular Weight	972.2 g/mol
Synonyms	Cy5-PEG3-Azide-PEG3-amine; N-(Azido-PEG3)-Cy5-PEG3-amine; Cyanine5 bis-PEG3 azide amine; 1-Azido-3,6,9,12,15,18,21-heptaoxatetratriacontan-34-amine, N-[2-[2-[2-[2-[5-[(1E,3E)-5-[(1E,3E)-3-(1,3-Dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-1-propen-1-yl]-1,3-pentadien-1-yl]-1,3,3-trimethyl-3H-indolium-2-yl]-1-oxopropoxy]ethoxy]ethyl]carbamoyl]ethyl]-; Azide-PEG3-Cy5-PEG3-NH2; N3-PEG3-Cy5-PEG3-NH2
EINECS	Contact for details

Quality Control

Our n-Peg3-N'-(Azide-Peg3)-Cy5 is manufactured under strict quality protocols to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) with detailed spectral data (NMR, MS) are provided to guarantee compliance with your specific research or development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere (argon or nitrogen) and allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.