Description

5-Bromo-4-Chloro-3-Indolyl B-D-Lactopyranoside is a specialized chromogenic substrate used in enzymatic assays and diagnostic applications. This compound is critical for the sensitive detection and quantification of β-D-galactosidase activity, a key reporter enzyme in molecular biology and microbiology. It is an essential reagent for researchers and manufacturers in the fields of life science research, clinical diagnostics, and pharmaceutical development.

Application

• Enzymatic Reporter Assays: As a chromogenic substrate for detecting β-D-galactosidase activity in molecular biology, including applications in gene expression studies (e.g., lacZ reporter systems).
• Microbiological Testing: Used in media for the identification and differentiation of bacterial species (e.g., E. coli) based on galactosidase production.
• Diagnostic Kits: A key component in clinical and veterinary diagnostic kits for enzyme-linked detection methods.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology research for screening compound libraries against enzymatic targets.
• Histochemistry & Cell Biology: Used in staining protocols to localize β-D-galactosidase activity in tissues and cells.
• Food & Environmental Safety Testing: Applied in assays to detect specific microbial contaminants in food, water, and environmental samples.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl B-D-Lactopyranoside
CAS No.	2200269-31-2
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	X-Galactopyranoside; 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside; BCIG; X-β-D-Gal; 5-Bromo-4-chloro-1H-indol-3-yl β-D-galactopyranoside; Chromogenic Galactosidase Substrate; Indoxyl-β-D-galactoside derivative
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl B-D-Lactopyranoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to ISO 9001 quality management standards, and our processes are designed to meet the rigorous requirements of the life science industry.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Biological Activity	Conforms to performance standard in β-D-galactosidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.