Description

(4E)-Tco-Peg4-Biotin is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation and click chemistry applications. Its core value lies in the orthogonal reactivity of the trans-cyclooctene (TCO) group for rapid, bioorthogonal tetrazine ligation, coupled with the biotin moiety for powerful streptavidin-based detection or purification. This compound is essential for researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development who require precise and stable labeling of biomolecules.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads or fluorescent probes to monoclonal antibodies via TCO-tetrazine click chemistry.
• Cell Surface Labeling & Imaging: Modification of cell surface proteins or glycans with biotin tags for visualization or isolation using streptavidin reagents.
• Protein & Peptide Modification: Introduction of a biotin handle onto proteins, peptides, or other biomolecules for pull-down assays, ELISA, or Western blot detection.
• Pre-Targeting Strategies: Utilized in multi-step pre-targeting approaches for radioimmunotherapy or imaging, where the TCO-modified targeting vector is administered before the radiolabeled tetrazine.
• Biomaterial Functionalization: Grafting of bioactive peptides or proteins onto nanoparticles, hydrogels, or other material surfaces for targeted delivery or cell culture applications.
• Diagnostic Probe Synthesis: Construction of sensitive detection probes by conjugating recognition elements (e.g., antibodies, oligonucleotides) to reporter systems via the PEG4 spacer.

Basic Information

Product Name	(4E)-Tco-Peg4-Biotin
CAS No.	2183440-30-2
Molecular Formula	C₃₃H₅₇N₅O₈S
Molecular Weight	683.90 g/mol
Synonyms	(4E)-TCO-PEG4-Biotin; TCO-PEG4-Biotin; (E)-TCO-PEG4-Biotin; trans-Cyclooctene-PEG4-Biotin; Biotin-PEG4-TCO; Biotin-PEG4-(4E)-TCO; 5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-[3-[(4E)-cyclooct-4-en-1-yloxy]propanoylamino]ethoxy]ethoxy]ethoxy]ethyl]pentanamide
EINECS	Contact for details

Quality Control

Our (4E)-Tco-Peg4-Biotin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry to ensure identity and consistency. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.