Description

Dnp-Peg4-Dbco is a heterobifunctional crosslinker featuring a 2,4-dinitrophenyl (DNP) group and a dibenzocyclooctyne (DBCO) moiety connected by a tetraethylene glycol (PEG4) spacer. This compound is engineered for efficient, copper-free click chemistry applications, enabling the specific labeling and conjugation of biomolecules under mild, physiological conditions. It is an essential reagent for researchers and developers in bioconjugation, proteomics, and the development of antibody-drug conjugates (ADCs), where precise and stable linkage is critical.

Application

• Bioconjugation and Protein Labeling: Used to attach fluorescent dyes, drugs, or other probes to proteins, antibodies, or peptides via the DBCO-azide cycloaddition.
• Antibody-Drug Conjugate (ADC) Development: Serves as a key linker-payload component for constructing stable ADCs, leveraging the DNP group for specific attachment and the DBCO for bioorthogonal coupling.
• Surface Functionalization: Enables the modification of nanoparticles, biosensors, and microarray surfaces with specific ligands or capture agents.
• Chemical Biology and Proteomics: Facilitates the study of protein-protein interactions, cellular trafficking, and target identification through site-specific labeling strategies.
• Diagnostic Assay Development: Employed in the creation of sensitive immunoassays and diagnostic kits that require stable, high-affinity conjugates.
• Polymer and Material Science: Acts as a functional crosslinker for synthesizing advanced polymeric materials with tailored biological activity.

Basic Information

Item	Details
Product Name	Dnp-Peg4-Dbco
CAS No.	2149585-17-9
Molecular Formula	C₃₃H₄₀N₄O₁₁
Molecular Weight	668.69 g/mol
Synonyms	2,4-Dinitrophenyl-PEG4-DBCO; DBCO-PEG4-DNP; DNP-PEG4-Dibenzocyclooctyne; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (2,5,8,11-tetraoxatetradecan-14-yl) (2,4-dinitrophenyl)carbamate; DNP-PEG4-Dibenzylcyclooctyne; DNP-PEG4-DBCO Crosslinker; DNP-PEG₄-DBCO; Heterobifunctional PEG linker DNP-PEG4-DBCO
EINECS	Contact for details

Quality Control

Our Dnp-Peg4-Dbco is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for research and development. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability and consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.