Description

(+)-Biotin-Peg8-Nhs Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for researchers and manufacturers requiring precise, stable, and site-specific labeling of biomolecules, particularly in sensitive assay development. It is primarily needed by professionals in the pharmaceutical, diagnostic, and life science research sectors for creating biotinylated probes, antibody-drug conjugates (ADCs), and targeted delivery systems.

Application

• Bioconjugation & Probe Development: Site-specific labeling of antibodies, proteins, peptides, and oligonucleotides with biotin for detection and purification.
• Diagnostic Assay Fabrication: Key reagent for developing ELISA, lateral flow assays, and other immunoassays by conjugating detection molecules to solid supports.
• Targeted Drug Delivery Systems: Construction of antibody-drug conjugates (ADCs) and other ligand-directed therapeutics utilizing the PEG8 spacer for improved solubility and pharmacokinetics.
• Affinity Chromatography: Preparation of biotinylated ligands for immobilization on streptavidin/avidin resins.
• Cell Surface Engineering: Labeling of cell membrane proteins for imaging, tracking, and sorting applications (e.g., flow cytometry).
• Microarray & Biosensor Development: Functionalization of surfaces with bioactive molecules for high-throughput screening and sensing platforms.

Basic Information

Product Name	(+)-Biotin-Peg8-Nhs Ester
CAS No.	2143968-03-8
Molecular Formula	C₄₀H₆₈N₄O₁₇S
Molecular Weight	925.04 g/mol
Synonyms	Biotin-PEG8-NHS ester; Biotin-PEG8-NHS; Biotin-PEG8-SSE; Biotinamidohexanoyl-PEG8-NHS Ester; Biotin-PEG₈-NHS Ester; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2,5,8,11,14,17,20,23-octaoxapentacosan-25-yl)pentanamide 2,5-Dioxopyrrolidin-1-yl ester; Biotinylated PEG NHS ester (PEG8 linker)
EINECS	Contact for details

Quality Control

Our (+)-Biotin-Peg8-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (NHS ester content)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.