Description

Tco4-Peg3-Nhs CAS NO 2141981-88-4 is a heterobifunctional crosslinking reagent featuring a tetrazine cyclooctyne (TCO) group and an N-hydroxysuccinimide (NHS) ester, connected by a triethylene glycol (PEG3) spacer. This compound is essential for bioorthogonal click chemistry, enabling rapid, specific, and catalyst-free conjugation between biomolecules under physiological conditions. It is primarily used by researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and the synthesis of diagnostic probes and targeted therapeutics.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via the TCO-tetrazine reaction.
• Biomolecule Labeling & Imaging: Fluorescent or radioactive labeling of proteins, peptides, and oligonucleotides for in vitro and in vivo tracking.
• Surface Functionalization: Modification of nanoparticles, biosensors, and microarray surfaces with bioactive ligands for enhanced targeting and detection.
• Proteomics Research: Chemical proteomics applications for studying protein-protein interactions and post-translational modifications.
• Diagnostic Reagent Development: Creation of sensitive immunoassay components and molecular imaging probes.
• Polymer & Material Science: Synthesis of functionalized hydrogels and smart biomaterials with controlled bioactivity.

Basic Information

Item	Details
Product Name	Tco4-Peg3-Nhs
CAS No.	2141981-88-4
Molecular Formula	C₃₀H₄₀N₄O₉
Molecular Weight	600.66 g/mol
Synonyms	trans-Cyclooctene-PEG3-NHS ester; TCO-PEG3-NHS ester; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-((2,5-dioxopyrrolidin-1-yl)oxy)-1-oxo-3,6,9,12-tetraoxapentadecan-15-yl) carbamate; TCO-PEG3-NHS; TCO-(PEG)3-NHS Ester; TCO-PEG(3)-NHS; TCO-PEG₃-NHS
EINECS	Contact for details

Quality Control

Our Tco4-Peg3-Nhs is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by NMR)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.