Description

Tetrazine-Ss-Biotin is a high-purity, heterobifunctional chemical linker designed for advanced bioconjugation and labeling applications. This compound is engineered to enable rapid, bioorthogonal click chemistry through its stable tetrazine moiety while providing a biotin handle for subsequent purification or detection via streptavidin. It is an essential reagent for researchers and developers in the pharmaceutical and diagnostic sectors who require precise, efficient, and stable conjugation strategies. The product is supplied to meet the stringent demands of modern life science research and biopharmaceutical development.

Application

• Bioconjugation Platform: For site-specific labeling of antibodies, proteins, peptides, and oligonucleotides with biotin.
• Pretargeted Imaging & Therapy: Enables inverse electron-demand Diels-Alder (IEDDA) click chemistry for pretargeted radioimmunotherapy and PET/MRI imaging probe construction.
• Diagnostic Assay Development: Used to create sensitive detection systems in ELISA, lateral flow assays, and other immunoassays by conjugating detection molecules.
• Affinity Purification: Facilitates the capture and isolation of tetrazine-labeled biomolecules using streptavidin-coated beads or columns.
• Chemical Biology Probes: Serves as a key building block for creating activity-based probes to study enzyme function or cellular processes.
• ADC (Antibody-Drug Conjugate) Research: Explored as a linker component in novel ADC architectures for controlled payload delivery.
• Surface Functionalization: For immobilizing biomolecules onto biosensors, microarrays, and other solid supports in a controlled orientation.
• Materials Science: Incorporation into polymers or nanomaterials to create biofunctionalized materials with specific targeting capabilities.

Basic Information

Item	Details
Product Name	Tetrazine-Ss-Biotin
CAS No.	2123482-78-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Biotin-PEG4-Tetrazine; Biotin-PEG4-s-Tetrazine; Tetrazine-PEG4-Biotin; s-Tetrazine-PEG4-Biotin; Biotin-(PEG)4-Tetrazine; 1,2,4,5-Tetrazine-PEG4-Biotin Conjugate; 3,6-Bis-(PEG4-biotin)-1,2,4,5-tetrazine derivative; Biotinylated Tetrazine Linker
EINECS	Contact for details

Quality Control

Our Tetrazine-Ss-Biotin is manufactured under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and structural confirmation via mass spectrometry (MS) and NMR. We provide a Certificate of Analysis (COA) with every shipment, detailing key specifications such as purity, identity, and solvent content. Our quality commitment ensures the reproducibility and reliability required for critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.