Description

n-Mal-n-Bis(Peg4-Nhs Ester) is a heterobifunctional crosslinking reagent featuring a maleimide group and an amine-reactive N-hydroxysuccinimide (NHS) ester, connected by a tetraethylene glycol (PEG4) spacer. This compound is engineered for the site-specific conjugation of biomolecules, enabling the creation of stable thioether bonds with cysteine residues while minimizing steric hindrance and improving solubility. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay formulation, where precise and reproducible linking chemistry is critical.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the controlled attachment of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein and Peptide Labeling: Site-specific conjugation of fluorophores, biotin, or other probes to proteins for imaging and detection.
• Surface Functionalization: Immobilization of proteins, peptides, or other ligands onto biosensor chips, nanoparticles, or solid supports.
• Diagnostic Reagent Synthesis: Production of enzyme conjugates and labeled antibodies for ELISA, lateral flow assays, and other immunoassays.
• Polymer and Biomaterial Modification: Introducing bioactive molecules into hydrogels or polymer scaffolds for tissue engineering and drug delivery systems.
• Stable Cell-Penetrating Peptide Conjugates: Creating cell-targeting constructs with enhanced stability and bioavailability.

Basic Information

Product Name	n-Mal-n-Bis(Peg4-Nhs Ester)
CAS No.	2112738-60-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Maleimide-PEG4-NHS ester; NHS-PEG4-Maleimide; SM(PEG)4; Maleimido tetraoxatridecyl succinimidyl ester; 3,6,9,12-Tetraoxapentacos-24-ene-1,25-dioic acid bis(2,5-dioxopyrrolidin-1-yl) ester; PEG4 crosslinker; Heterobifunctional PEG linker, Mal-PEG4-NHS
EINECS	Contact for details

Quality Control

Our n-Mal-n-Bis(Peg4-Nhs Ester) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC purity analysis, NMR structural confirmation, and functional group assay, to ensure consistency and performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are provided to guarantee traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (H-NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Functional Group Assay (Maleimide)	≥ 90%
Functional Group Assay (NHS Ester)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.