Description

n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Azide-Peg4)-Cy3 is a sophisticated, bifunctional fluorescent labeling reagent designed for advanced bioconjugation and bioimaging applications. This compound integrates a cyanine 3 (Cy3) fluorophore with a hydrophilic polyethylene glycol (PEG) spacer and a reactive azide group, enabling efficient, site-specific labeling via click chemistry. It is essential for researchers and developers in pharmaceutical discovery, diagnostic assay development, and advanced materials science who require precise, stable, and bright fluorescent probes for tracking biomolecules.

Application

• Bioconjugation and Probe Development: Site-specific labeling of antibodies, proteins, peptides, and oligonucleotides for fluorescence-based assays (e.g., ELISA, Western blot, flow cytometry).
• Live-Cell and In Vivo Imaging: Conjugation to targeting ligands (e.g., peptides, small molecules) for real-time visualization of cellular processes and biodistribution studies.
• Diagnostic Kit Manufacturing: Production of fluorescent lateral flow assays, microarray chips, and other point-of-care diagnostic devices.
• Polymer and Materials Science: Functionalization of nanoparticles, hydrogels, and polymeric materials to create traceable, smart delivery systems or biosensors.
• Chemical Biology & Proteomics: As a critical tool for activity-based protein profiling (ABPP) and studying protein-protein interactions.
• Drug Discovery & Development: Labeling of drug candidates for pharmacokinetic, pharmacodynamic, and ADME (Absorption, Distribution, Metabolism, and Excretion) studies.

Basic Information

Product Name	n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Azide-Peg4)-Cy3
CAS No.	2107273-64-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy3-PEG4-Azide; Azido-PEG4-Cy3; N-Methyl-N'-methyl-O-(m-PEG4)-O'-(azido-PEG4)-Cy3; Cyanine3 azide PEG4; Azide-PEG4-Cyanine3; Click Chemistry Cy3 Reagent; Fluorescent PEG Azide; 3-(N-Methyl-N-(3-(N-Methyl-N-(3-(2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethoxy)propyl)amino)propyl)amino)propyl)-2-((1E,3E)-3-(1-ethyl-3,3-dimethylindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-1-propyl-3H-indol-1-ium
EINECS	Contact for details

Quality Control

Our n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Azide-Peg4)-Cy3 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and functional validation of the azide reactivity and fluorescence properties. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research and production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient	~150,000 cm⁻¹M⁻¹ (at λmax ~550 nm)
Functional Group (Azide)	Positive by IR or functional assay
Moisture Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, methanol, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.