Description

n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Propargyl-Peg4)-Cy3 is a specialized, bifunctional cyanine dye derivative engineered for advanced bioconjugation and labeling applications. Its core value lies in the combination of a Cy3 fluorophore for bright, stable emission with dual functional handles—a propargyl group for copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry and a methoxy-terminated PEG4 spacer for enhanced solubility and reduced non-specific binding. This product is essential for researchers and developers in biopharmaceuticals, diagnostic assay development, and advanced materials science who require precise, site-specific fluorescent labeling of biomolecules such as proteins, antibodies, and oligonucleotides.

Application

• Site-Specific Bioconjugation: Fluorescent labeling of proteins, peptides, and antibodies via click chemistry for flow cytometry, microscopy (FRET), and immunoassays.
• Nucleic Acid Labeling: Modification of oligonucleotides, DNA, or RNA probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Drug Delivery & Theranostics: Conjugation to drug carriers, nanoparticles, or targeting ligands for imaging-tracked delivery systems.
• Biomaterial Functionalization: Incorporation into hydrogels, polymer scaffolds, or surface coatings to create fluorescently tagged materials for cell culture and tissue engineering studies.
• Diagnostic Reagent Development: A key component in the synthesis of fluorescent tracers and detection reagents for clinical diagnostic kits and biosensors.
• Chemical Biology Probes: Synthesis of activity-based probes (ABPs) or photoaffinity labels for target identification and enzyme activity profiling.

Basic Information

Product Name	n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Propargyl-Peg4)-Cy3
CAS No.	2107273-62-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cy3 alkyne PEG4 methyl ether; Propargyl-PEG4-Cy3 methyl ether; Cyanine3 alkyne PEG4 O-methyl; m-PEG4-Cy3-Propargyl; N-Methyl-N'-methyl Cy3 PEG4 alkyne; Click chemistry Cy3 dye with PEG spacer; Bifunctional Cy3-PEG4-alkyne dye
EINECS	Contact for details

Quality Control

Every batch of n-Methyl-N'-Methyl-o-(m-Peg4)-O'-(Propargyl-Peg4)-Cy3 is manufactured under strict quality protocols. Our products undergo rigorous quality testing, including HPLC for purity and identity confirmation, to ensure compliance with research-grade and cGMP standards for advanced bioconjugation applications. Comprehensive Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term storage, keep the container in a freezer and under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.