Description

n-(m-Peg4)-N'-(Peg4-Nhs Ester)-Cy5 is a heterobifunctional, hydrophilic cyanine dye derivative designed for advanced bioconjugation and labeling applications. This compound matters for its dual functionality, combining a maleimide group for thiol-selective coupling with an NHS ester for amine-reactive labeling, all linked via flexible polyethylene glycol (PEG) spacers to enhance solubility and reduce steric hindrance. It is primarily needed by researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced life science research for creating stable, fluorescently tagged biomolecules.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific labeling of antibodies via cysteine residues for targeted therapy research.
• Protein and Peptide Labeling: Fluorescent tagging of proteins, peptides, and other biomolecules for detection and analysis.
• Biomolecular Imaging Probes: Synthesis of near-infrared (NIR) fluorescent probes for in vitro and in vivo imaging applications.
• Diagnostic Reagent Manufacturing: Conjugation to detection antibodies or antigens in ELISA, lateral flow assays, and other diagnostic platforms.
• Cell Surface Labeling: For tracking and visualization of cell surface proteins and receptors in flow cytometry and microscopy.
• Polymer and Nanoparticle Functionalization: Introducing Cy5 fluorescence to polymers, dendrimers, or nanoparticles for traceability and biodistribution studies.
• Nucleic Acid Detection Systems: Labeling of oligonucleotide probes for fluorescence in situ hybridization (FISH) and real-time PCR.
• Biomaterial and Hydrogel Research: Incorporating fluorescent markers into scaffolds and materials for biomedical engineering.

Basic Information

Item	Detail
Product Name	n-(m-Peg4)-N'-(Peg4-Nhs Ester)-Cy5
CAS No.	2107273-30-1
Molecular Formula	C73H103N5O22
Molecular Weight	~1410.6 g/mol
Synonyms	Cy5 maleimide-PEG4-NHS ester; Maleimide-PEG4-Cy5-PEG4-NHS ester; Cy5-PEG4-MAL-PEG4-NHS; Cy5 NHS ester-PEG4-maleimide; Sulfo-Cy5 maleimide-PEG4-NHS ester analog; N-(3-Maleimidopropionyl-PEG4)-N'-(PEG4-N-succinimidyl ester)-Cy5; Cy5 bifunctional PEG linker; Heterobifunctional Cy5-PEG crosslinker
EINECS	Contact for details

Quality Control

Our n-(m-Peg4)-N'-(Peg4-Nhs Ester)-Cy5 is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and batch-to-batch reproducibility suitable for sensitive research and development applications. Certificates of Analysis (COA) detailing purity, identity, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~250,000 L·mol⁻¹·cm⁻¹ (at λmax ~649 nm)
Solubility	Soluble in DMSO, DMF, acetonitrile, water
Functional Group Integrity	≥ 90% (by functional assay)
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.