Description

n-(Amino-Peg4)-n-Biotin-Peg4-Acid is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a biotin moiety and a terminal carboxylic acid, designed for advanced bioconjugation and labeling applications. This compound is critical for researchers and developers requiring precise, stable, and high-affinity linkages in complex molecular systems. It is primarily utilized in the pharmaceutical, diagnostic, and life science research sectors for creating targeted drug delivery systems, diagnostic probes, and affinity purification matrices.

Application

• ADC (Antibody-Drug Conjugate) Development: As a versatile linker for attaching cytotoxic payloads to monoclonal antibodies, enabling targeted cancer therapies.
• Diagnostic Assay Fabrication: For immobilizing proteins, antibodies, or nucleic acids onto streptavidin-coated surfaces in ELISA, biosensors, and microarray platforms.
• Affinity Chromatography & Purification: Functionalizing solid supports to create resins for the selective capture and purification of biotinylated biomolecules.
• Fluorescent & Imaging Probe Synthesis: Conjugating biotin to dyes, nanoparticles, or other reporter molecules for use in fluorescence microscopy, flow cytometry, and in vivo imaging.
• Protein & Peptide Modification: Site-specific biotinylation of proteins via the amine-reactive PEG spacer for studies in protein-protein interactions, pull-down assays, and structural biology.
• Nanoparticle Functionalization: Coating liposomes, polymeric nanoparticles, or quantum dots to impart targeting capabilities and stealth properties via the PEG chain.
• Surface Plasmon Resonance (SPR): Immobilizing ligands on sensor chips for real-time analysis of biomolecular interactions.
• Precision Medicine Research: Developing novel theranostic agents that combine therapeutic and diagnostic functions within a single molecule.

Basic Information

Product Name	n-(Amino-Peg4)-n-Biotin-Peg4-Acid
CAS No.	2100306-84-9
Molecular Formula	C41H75N5O15S
Molecular Weight	910.13 g/mol
Synonyms	Biotin-PEG4-acid-PEG4-amine; Biotin-PEG4-CH2CH2COOH-PEG4-NH2; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-(2-(2-(2-(2-(2-aminoethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)pentanamide; Amino-PEG4-Biotin-PEG4-acid; Biotin-PEG4-COOH-PEG4-NH2; Biotinylated Heterobifunctional PEG Crosslinker; Biotin-PEG4-acid, amine terminated; N-(Amino-PEG4)-N'-(Biotin-PEG4)-acid
EINECS	Contact for details

Quality Control

Every batch of n-(Amino-Peg4)-n-Biotin-Peg4-Acid is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and consistency. Our products are suitable for research and development use under cGMP guidelines where applicable. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming specifications for assay (HPLC), purity, and functional group integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by weight)
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines
Functional Group Analysis	Amine & Acid content within theoretical range

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.