Description

n-(Azido-Peg3)-n-Fluorescein-Peg3-Acid is a heterobifunctional crosslinker and labeling reagent that combines a click-reactive azide group with a fluorescent fluorescein moiety, connected by flexible, hydrophilic polyethylene glycol (PEG) spacers. This compound is essential for advanced bioconjugation strategies, enabling the fluorescent tagging of biomolecules via bioorthogonal click chemistry for sensitive detection and imaging. It is primarily utilized by researchers and developers in biopharmaceutical R&D, diagnostic assay development, and materials science for creating labeled probes, studying biomolecular interactions, and developing novel therapeutic conjugates.

Application

• Bioconjugation and Probe Development: Fluorescent labeling of antibodies, proteins, peptides, and oligonucleotides for use in immunoassays (ELISA, Western Blot) and fluorescence in situ hybridization (FISH).
• Cell Imaging and Tracking: Labeling of cell surface glycans, lipids, or other biomolecules for real-time visualization and tracking in live-cell or fixed-cell microscopy.
• Drug Delivery & ADC Research: Conjugation to drug molecules or nanoparticles to create traceable drug delivery systems and study the pharmacokinetics of antibody-drug conjugates (ADCs).
• Materials Science & Surface Functionalization: Modification of polymers, hydrogels, and nanoparticles with fluorescent tags for biosensor development and biomaterial characterization.
• Click Chemistry Reagent: Serving as a critical reagent in copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC) reactions.
• Diagnostic Kit Manufacturing: Production of key components for lateral flow assays, fluorescence-based point-of-care tests, and other diagnostic platforms.

Basic Information

Product Name	n-(Azido-Peg3)-n-Fluorescein-Peg3-Acid
CAS No.	2100306-50-9
Molecular Formula	C44H55N5O16
Molecular Weight	910.0 g/mol (approx.)
Synonyms	5-FAM-PEG3-Azide; 5-Carboxyfluorescein-PEG3-Azide; Azido-PEG3-Fluorescein-PEG3-acid; 6-(2-(2-(3-Azidopropoxy)ethoxy)ethoxy)-N-(2-(2-(2-(2,4-dihydroxy-3-oxo-3H-xanthen-6-yl)benzoyl)amino)ethoxy)ethoxy)ethyl)-6-oxohexanamide; Fluorescein-PEG3-N3; Azido-PEG3-5-FAM; 5-FAM Azido-PEG3 acid; Azido-PEG3-Carboxyfluorescein
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg3)-n-Fluorescein-Peg3-Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and batch-to-batch reproducibility, critical for sensitive research and development applications. Certificates of Analysis (COA) detailing purity, identity, and functional performance are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~492 nm
Emission Maximum	~518 nm
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.