Description

Azadibenzocyclooctyne-Peg12-n-Hydroxysuccinimidyl Ester is a high-purity, heterobifunctional crosslinker designed for bioorthogonal conjugation chemistry. This compound is critical for enabling stable, copper-free click chemistry reactions between azide-labeled biomolecules and other functionalized surfaces or probes. It is primarily needed by researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced materials science for creating next-generation diagnostic tools, targeted therapeutics, and functional biomaterials.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Site-specific labeling of antibodies, proteins, and peptides with fluorophores, drugs, or other payloads via its NHS ester and DBCO groups.
• Proteomics and Biomarker Discovery: Immobilization of azide-tagged proteins or glycans onto DBCO-functionalized solid supports for microarray or pull-down assays.
• In Vivo Imaging and Diagnostics: Conjugation of targeting ligands (e.g., peptides, antibodies) to imaging agents (e.g., near-infrared dyes, radionuclide chelators) for preclinical studies.
• Polymer and Biomaterial Functionalization: Modification of hydrogels, nanoparticles, or surfaces with bioactive molecules to create smart materials for tissue engineering or drug delivery.
• Nucleic Acid Labeling: Attachment of reporter molecules to azide-modified oligonucleotides for FISH, sequencing, or detection applications.
• Small Molecule Probe Synthesis: Construction of chemical probes for activity-based protein profiling (ABPP) or target identification.

Basic Information

Product Name	Azadibenzocyclooctyne-Peg12-n-Hydroxysuccinimidyl Ester
CAS No.	2093934-94-0
Molecular Formula	C58H88N4O27
Molecular Weight	1297.3 g/mol
Synonyms	ADIBO-PEG12-NHS Ester; DBCO-PEG12-NHS Ester; Azadibenzocyclooctyne-PEG12-NHS Ester; 1-(12-((1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethoxy)-12-oxododeca-3,6,9-trioxa-1-azadodecyl)-2,5-dioxopyrrolidine-3-carboxylate; DBCO-SS-PEG12-NHS; DBCO-PEG12-N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our Azadibenzocyclooctyne-Peg12-n-Hydroxysuccinimidyl Ester is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive research and development applications. Each lot undergoes comprehensive analytical characterization. Certificates of Analysis (COA) providing detailed results for purity, identity, and functional integrity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (NHS Ester)	≥ 90% active ester

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.