Description

2-(Biotin-Amido)-1,3-Bis(Carboxylethoxy)Propane is a specialized, high-purity biotin derivative designed for advanced bioconjugation and linker chemistry. This compound matters for researchers and manufacturers requiring precise, stable attachment of biotin to biomolecules or surfaces for detection, purification, and diagnostic applications. It is essential for biotech, pharmaceutical R&D, and diagnostic kit producers developing sensitive assay platforms like ELISA, lateral flow tests, and affinity chromatography systems.

Application

• Diagnostic Assay Development: As a key linker for immobilizing capture antibodies or antigens on solid supports (e.g., microplates, beads, membranes) in ELISA, lateral flow assays, and other immunoassays.
• Affinity Purification Systems: Functionalization of resins and matrices (e.g., agarose, magnetic beads) for the purification of streptavidin/avidin-tagged proteins, nucleic acids, or other biomolecules.
• Bioconjugation & Probe Synthesis: Serving as a heterobifunctional crosslinker for labeling proteins, peptides, antibodies, or oligonucleotides with biotin for detection and imaging studies.
• Biosensor & Microarray Fabrication: Creating stable, high-density biotinylated surfaces on chips and sensors to capture target analytes via streptavidin-biotin interaction.
• Cell Biology & Microscopy: Conjugation to ligands or other molecules for studying cell surface receptors, trafficking, and other processes using fluorescent streptavidin probes.
• Drug Delivery & Targeting: Potential use in constructing biotinylated drug conjugates or nanocarriers for targeted delivery systems leveraging overexpressed biotin receptors on certain cell types.

Basic Information

Item	Details
Product Name	2-(Biotin-Amido)-1,3-Bis(Carboxylethoxy)Propane
CAS No.	2086689-02-1
Molecular Formula	C22H35N3O9S
Molecular Weight	517.59 g/mol
Synonyms	2-(Biotinamido)-1,3-bis(carboxyethoxy)propane; Biotin-PEG2-COOH; Biotin-PEG2-acid; Biotin-amido-PEG2-acid; Biotin-PEG2-acetic acid; Biotin-ethyleneglycol-acetic acid; N-Biotinyl-3,6-dioxaoctanedioic acid; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[2-[2-(carboxymethoxy)ethoxy]ethyl]pentanamide
EINECS	Contact for details

Quality Control

Our 2-(Biotin-Amido)-1,3-Bis(Carboxylethoxy)Propane is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccant or under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.