Description

Methyltetrazine-Peg12-Nhs Ester is a heterobifunctional crosslinking reagent that combines a bioorthogonal methyltetrazine group with a long, hydrophilic polyethylene glycol (PEG) spacer and an amine-reactive N-hydroxysuccinimide (NHS) ester. This molecular design is critical for creating stable, site-specific conjugates in aqueous biological systems while minimizing non-specific binding. It is an essential tool for researchers and developers in bioconjugation, ADC (Antibody-Drug Conjugate) development, and advanced materials science, enabling the precise labeling and functionalization of proteins, antibodies, and nanoparticles.

Application

• ADC (Antibody-Drug Conjugate) Linker Synthesis: For constructing stable, cleavable, or non-cleavable linkers between monoclonal antibodies and cytotoxic payloads.
• Protein and Antibody Labeling: Site-specific modification of lysine residues for attaching fluorophores, drugs, or other functional molecules via bioorthogonal click chemistry.
• Surface Functionalization: Modification of nanoparticles, biosensors, and diagnostic platforms to introduce tetrazine groups for subsequent click reactions.
• Pretargeted Imaging and Therapy: Enabling multi-step strategies in nuclear medicine and oncology where a tetrazine-modified targeting vector reacts with a radiolabeled or therapeutic trans-cyclooctene (TCO) agent in vivo.
• Polymer and Biomaterial Conjugation: Incorporating bioorthogonal reactivity into hydrogels, scaffolds, and other polymeric materials for controlled drug release or cell interaction studies.
• Diagnostic Probe Development: Creating sensitive and specific probes for ELISA, flow cytometry, and in vitro diagnostics (IVD).

Basic Information

Product Name	Methyltetrazine-Peg12-Nhs Ester
CAS No.	2055646-25-6
Molecular Formula	C58H96N6O28
Molecular Weight	~1349.4 g/mol
Synonyms	m-Tetrazine-PEG12-NHS ester; Methyltetrazine-PEG12-NHS ester; 5-Methyl-1,2,4-triazine-3,6-diamine-PEG12-NHS; Methyltetrazine-PEG12-N-hydroxysuccinimide ester; Methyltetrazine-Polyethylene glycol-NHS; MeTz-PEG12-NHS; Bioorthogonal Crosslinker 2055646-25-6; Tetrazine-PEG-NHS Ester (12 Unit Spacer)
EINECS	Contact for details

Quality Control

Our Methyltetrazine-Peg12-Nhs Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications like ADC development. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional integrity. We adhere to cGMP principles where applicable, and our quality system is designed to meet the stringent requirements of pharmaceutical R&D and preclinical studies.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. For long-term stability, store under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange viscous oil or solid
Identification (HPLC/MS)	Complies
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity (NHS Ester)	≥ 90%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.