Description

Biotin-Peg12-Tfp Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for researchers and manufacturers who require precise and stable attachment of biotin tags to biomolecules, enabling downstream detection, purification, and analysis. It is primarily utilized in the development of diagnostic assays, targeted drug delivery systems, and proteomics research within the pharmaceutical, biotechnology, and life science sectors.

Application

• Antibody-Drug Conjugate (ADC) Development: Used as a linker-payload component for creating targeted therapeutics with cleavable or non-cleavable biotin handles.
• Diagnostic Assay Fabrication: Conjugation of biotin to antibodies, proteins, or oligonucleotides for use in ELISA, lateral flow tests, and other immunoassays.
• Affinity Purification Systems: Immobilization of ligands onto solid supports (e.g., agarose beads) for streptavidin-based capture and purification.
• Cell Surface Labeling & Imaging: Biotinylation of cell surface proteins for visualization and tracking using fluorescent streptavidin conjugates.
• Proteomics & Protein Interaction Studies: Crosslinking agent for studying protein-protein interactions and mapping binding sites.
• Nanoparticle Functionalization: Surface modification of liposomes, polymers, or inorganic nanoparticles for targeted delivery applications.
• Biosensor Development: Creating sensitive detection layers by attaching biorecognition elements to sensor surfaces.

Basic Information

Product Name	Biotin-Peg12-Tfp Ester
CAS No.	2055105-33-2
Molecular Formula	C58H98N4O23S
Molecular Weight	1251.5 g/mol
Synonyms	Biotin-PEG12-TFP Ester; Biotin-PEG12-2,3,5,6-Tetrafluorophenyl Ester; 2,3,5,6-Tetrafluorophenyl 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoylamino)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate; TFP-PEG12-Biotin; Biotin-PEG12-Tetrafluorophenyl Ester; Biotinylated PEG TFP Ester
EINECS	Contact for details

Quality Control

Our Biotin-Peg12-Tfp Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry, to ensure it meets the high standards required for research and pharmaceutical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.