Description

Dde Biotin-Peg4-Picolyl Azide is a multifunctional chemical reagent designed for advanced bioconjugation and click chemistry applications. Its core value lies in integrating a cleavable Dde protecting group, a biotin affinity tag, a hydrophilic PEG4 spacer, and a picolyl azide moiety for copper-free click reactions. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, and diagnostic assay creation who require precise, multi-step labeling and purification strategies.

Application

• Proteomics and Protein Labeling: Site-specific modification of proteins and peptides for activity-based protein profiling (ABPP) and pull-down assays.
• Antibody-Drug Conjugate (ADC) Development: Facilitating the construction of cleavable linker-payload systems for targeted cancer therapies.
• Bioorthogonal Chemistry: Serving as a key reagent for strain-promoted azide-alkyne cycloaddition (SPAAC) in live-cell imaging and labeling.
• Affinity Purification Probes: Synthesis of biotinylated probes for streptavidin-based capture and isolation of biomolecular targets.
• Diagnostic Reagent Synthesis: Building blocks for developing sensitive ELISA and lateral flow assay components.
• Materials Science: Functionalization of nanoparticles and surfaces with bioactive molecules for biosensor applications.

Basic Information

Product Name	Dde Biotin-Peg4-Picolyl Azide
CAS No.	2055048-42-3
Molecular Formula	C₄₀H₆₂N₁₀O₁₀S
Molecular Weight	851.06 g/mol
Synonyms	1-(4,4-Dimethyl-2,6-dioxocyclohex-1-ylidene)-3-[2-[2-[2-[2-[2-[5-[(2-azidoethyl)(pyridin-2-ylmethyl)amino]-5-oxopentyl]amino]ethoxy]ethoxy]ethoxy]ethylamino]propyl-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide; Biotin-PEG4-Dde-Picolyl-Azide; Dde-Biotin-PEG4-Picolyl-N₃; N-(2-Azidoethyl)-N-(pyridin-2-ylmethyl)-5-[(3-{2-[2-(2-{2-[5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl]amino}ethoxy)ethoxy]ethoxy}ethyl)amino]-5-oxopentanamide bearing a 1-(4,4-dimethyl-2,6-dioxocyclohexylidene)ethyl group; Click Chemistry Biotin Reagent
EINECS	Contact for details

Quality Control

Our Dde Biotin-Peg4-Picolyl Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry to ensure identity and consistency. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee compliance with research and development standards. Custom synthesis and specific purity grades (e.g., >95%, >98%) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.