Description

T-Boc-n-Amido-Peg5-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a tert-butyloxycarbonyl (Boc)-protected amine at one terminus and an N-hydroxysuccinimide (NHS) ester at the other. This compound is engineered to enable controlled, stepwise bioconjugation, allowing for the introduction of a protected amine handle that can be deprotected under mild acidic conditions for subsequent reactions. It is primarily utilized by researchers and process developers in the pharmaceutical and biotechnology sectors for the synthesis of peptide-drug conjugates, antibody-drug conjugates (ADCs), and the functionalization of biomaterials and nanoparticles.

Application

• Bioconjugation and ADC Development: A key reagent for the site-specific modification of antibodies, proteins, and peptides to create stable, homogeneous conjugates for targeted therapeutics.
• PEGylation Linker: Used to introduce a hydrophilic PEG spacer with a protected amine, improving the solubility, stability, and pharmacokinetic profiles of bioactive molecules.
• Solid-Phase Peptide Synthesis (SPPS): Serves as a versatile building block for introducing a PEG chain and a protected amine functionality into synthetic peptides.
• Surface Functionalization: Enables the covalent attachment of biomolecules to surfaces, nanoparticles, or hydrogels via the NHS ester, leaving a deprotectable amine for further coupling.
• Crosslinking Agent: Facilitates the controlled crosslinking of amine-containing molecules in multi-step synthesis for creating complex macromolecular architectures.
• Prodrug Synthesis: Employed in the design of prodrugs where a therapeutic agent is linked via a cleavable PEG spacer to a targeting moiety.

Basic Information

Product Name	T-Boc-n-Amido-Peg5-Nhs Ester
CAS No.	2055040-78-1
Molecular Formula	C28H50N2O12
Molecular Weight	606.71 g/mol
Synonyms	1-Boc-amino-3,6,9,12,15-pentaoxaheptadecan-17-oic Acid N-Succinimidyl Ester; Boc-NH-PEG5-NHS Ester; tert-Butyl (17-((2,5-dioxopyrrolidin-1-yl)oxy)-17-oxo-3,6,9,12,15-pentaoxaheptadecyl)carbamate; 2,5-Dioxopyrrolidin-1-yl 1-[(1,1-dimethylethoxy)carbonyl]amino-3,6,9,12,15-pentaoxaheptadecan-17-oate; Boc-Amine-PEG5-NHS; Boc-N-amido-PEG5-NHS; Boc-NH-PEG5-NHS Ester; α-Boc-amino-ω-NHS-PEG5
EINECS	Contact for details

Quality Control

Our T-Boc-n-Amido-Peg5-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and Mass Spectrometry, to ensure it meets the high standards required for research and bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.