Description

Fluorescein-Peg6-Acid CAS NO 2055014-69-0 is a high-purity, heterobifunctional fluorescent labeling reagent featuring a fluorescein fluorophore, a hydrophilic hexaethylene glycol (PEG6) spacer, and a carboxylic acid terminus. This molecular architecture is engineered to provide excellent water solubility and reduced non-specific binding, making it a critical tool for creating stable, fluorescent bioconjugates. It is primarily utilized by researchers and developers in the life sciences and diagnostics sectors for advanced biomolecular tagging and detection applications.

Application

• Bioconjugation and Probe Development: Used to label antibodies, peptides, proteins, and oligonucleotides with a bright, green-fluorescent tag for imaging and detection.
• Diagnostic Assay Reagent: Serves as a key component in the synthesis of fluorescent probes for ELISA, lateral flow assays, and other immunoassay platforms.
• Cell Imaging and Tracking: Enables the fluorescent labeling of biomolecules for real-time visualization and tracking in live-cell or fixed-cell microscopy.
• Drug Delivery Research: Employed to functionalize nanoparticles, liposomes, or polymer carriers with a traceable fluorescent marker to study biodistribution and pharmacokinetics.
• Biomaterial Functionalization: Used to introduce fluorescent properties onto surfaces, hydrogels, or scaffolds for material characterization and cell interaction studies.
• Flow Cytometry: Facilitates the creation of fluorescently-labeled ligands or antibodies for high-throughput cell analysis and sorting.

Basic Information

Product Name	Fluorescein-Peg6-Acid
CAS No.	2055014-69-0
Molecular Formula	C41H49NO15
Molecular Weight	795.83 g/mol
Synonyms	5(6)-Carboxyfluorescein-PEG6-acid; 6-(Fluorescein-5(6)-carboxamido)hexanoic acid-PEG6-acid; Fluorescein-PEG6-COOH; 5(6)-FAM-PEG6-acid; 6-[(3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(or 6)-yl)carboxamido]hexanoic acid-PEG6-acid; Fluorescein hexaethylene glycol carboxylic acid; Xanthene dye-PEG6-COOH
EINECS	Contact for details

Quality Control

Our Fluorescein-Peg6-Acid is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and functional testing for labeling efficiency. Certificates of Analysis (COA) detailing lot-specific results are provided to ensure traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption (λmax)	~492 nm
Emission (λmax)	~518 nm
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.