Description

5-Fam-Ll-37 (Scrambled) is a fluorescently labeled, scrambled variant of the LL-37 peptide, a key component of the human innate immune system. This modified peptide is a critical research tool for studying antimicrobial peptide (AMP) mechanisms, membrane interactions, and cellular uptake without the inherent biological activity of the native sequence. It is primarily utilized by researchers and developers in pharmaceutical R&D, biochemistry, and immunology for fluorescence-based assays, imaging studies, and as a control in experiments investigating host defense peptides.

Application

• Fluorescence Microscopy & Imaging: Used as a fluorescent probe to visualize peptide localization, cellular uptake, and membrane interaction dynamics in live or fixed cells.
• Biophysical Studies: Essential for investigating the kinetics and thermodynamics of peptide-lipid bilayer interactions using techniques like fluorescence correlation spectroscopy (FCS) or fluorescence resonance energy transfer (FRET).
• Control in Antimicrobial Research: Serves as a crucial scrambled-sequence control in experiments to distinguish the specific effects of LL-37's primary structure from non-sequence-specific interactions.
• Drug Delivery Research: Investigated as a model for cell-penetrating peptides (CPPs) to study mechanisms for enhancing the delivery of therapeutic cargoes.
• High-Throughput Screening (HTS): Employed in assay development for screening libraries of compounds that modulate peptide-membrane interactions.
• Diagnostic Assay Development: Potential use in developing fluorescence-based sensors or assays for research purposes related to innate immunity.

Basic Information

Product Name	5-Fam-Ll-37 (Scrambled)
CAS No.	2022972-73-0
Molecular Formula	C214H340N60O64S
Molecular Weight	4874.4 g/mol (theoretical)
Synonyms	5-Carboxyfluorescein-LL-37 (scrambled); FAM-LL-37 scrambled peptide; Scrambled LL-37 labeled with 5-FAM; Fluorescein-labeled scrambled cathelicidin LL-37; hCAP-18 (scrambled, FAM-labeled); 5-FAM-LL37 (scrambled); F-LL-37 (scrambled); LL-37 Fluorescent Control Peptide (scrambled)
EINECS	Contact for details

Quality Control

Our 5-Fam-Ll-37 (Scrambled) is produced under stringent conditions to ensure batch-to-batch consistency for research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity verification and mass spectrometry (MS) for identity confirmation. Certificates of Analysis (COA) detailing purity, peptide content, and analytical data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, consider aliquoting to minimize freeze-thaw cycles and exposure to atmospheric moisture. Allow the vial to warm to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Single-Charge Mass (MALDI-TOF MS)	4874.4 ± 2.0 Da
Solubility	Soluble in water, DMSO, or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.