Description

(Lys(Me)32)-Histone H3 (21-44)-Gly-Lys(Biotinyl) is a chemically modified histone H3 peptide fragment, a critical research tool for studying epigenetic mechanisms and chromatin biology. This compound is specifically designed for investigating lysine methylation and biotinylation interactions, offering precise control in biochemical assays. It is essential for researchers and developers in pharmaceutical discovery, academic research, and diagnostic kit development focused on epigenetics and post-translational modifications.

Application

• Epigenetics Research: Used as a substrate or binding partner in assays studying histone methylation marks, particularly H3K9me3, and their role in gene regulation.
• Drug Discovery Screening: Serves as a target for high-throughput screening (HTS) to identify inhibitors or modulators of histone methyltransferases (HMTs) or reader proteins.
• Biochemical Assay Development: Employed in pull-down assays, ELISA, or surface plasmon resonance (SPR) to study protein-protein interactions within chromatin complexes.
• Antibody Validation: Acts as a specific antigen for the production and validation of antibodies against trimethylated lysine 9 on histone H3 (H3K9me3).
• Diagnostic Probe Development: Utilized in the development of probes for detecting specific epigenetic modifications in clinical research settings.
• Mass Spectrometry Standards: Functions as a calibrated standard in proteomics and mass spectrometry for the accurate quantification of modified peptides.

Basic Information

Product Name	(Lys(Me)32)-Histone H3 (21-44)-Gly-Lys(Biotinyl)
CAS No.	2022956-67-6
Molecular Formula	C₁₃₀H₂₂₈N₄₄O₃₈S
Molecular Weight	2986.5 g/mol (theoretical)
Synonyms	H3 (21-44) K9me3 Biotinylated Peptide; Histone H3 (21-44) (K9-trimethyl, K32-biotin) peptide; Biotinyl-Lys(Me)3-Histone H3(21-44); H3K9me3 Biotinylated Peptide Substrate; ARTKQTARK(me3)STGGKAPRKQLASK(Biotin)G; Epigenetic Peptide Substrate; Modified Histone Peptide; Chromatin Research Peptide
EINECS	Contact for details

Quality Control

Every batch of our (Lys(Me)32)-Histone H3 (21-44)-Gly-Lys(Biotinyl) is produced and analyzed under stringent conditions to ensure the highest standards of identity, purity, and biological activity. Our quality commitment includes comprehensive analytical characterization by HPLC, MS, and amino acid analysis. Certificates of Analysis (COA) detailing all specifications are provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated at -80°C. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Amino Acid Analysis	Within ± 10% of theoretical
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.