Description

(Lys(Ac)12)-Histone H4 (1-21)-Gly-Gly-Lys(Biotinyl) CAS NO 2022956-66-5 is a precisely modified, synthetic peptide fragment of histone H4, featuring extensive lysine acetylation and a C-terminal biotin tag. This compound is a critical research tool for studying epigenetic mechanisms, specifically histone modifications, protein-protein interactions, and chromatin biology. It is primarily utilized by academic, pharmaceutical, and biotechnology researchers focused on epigenetics, drug discovery, and diagnostic assay development.

Application

• Epigenetics Research: Used as a substrate or binding partner to study the role of histone H4 acetylation in gene regulation and chromatin remodeling.
• Biochemical Assay Development: Serves as a standardized component in high-throughput screening (HTS) assays for identifying modulators of histone acetyltransferases (HATs) or deacetylases (HDACs).
• Pull-down & Affinity Purification: The biotin tag enables efficient immobilization on streptavidin-coated surfaces for isolating interacting proteins or complexes from cell lysates.
• Antibody Validation & Specificity Testing: Acts as a positive control antigen for validating antibodies raised against acetylated histone H4.
• Structural Biology Studies: Provides a defined, modified histone peptide for NMR, X-ray crystallography, or other biophysical studies of modification-dependent molecular recognition.
• Diagnostic Kit Component: Can be incorporated into ELISA or other immunoassay formats for the quantitative detection of specific antibodies or reader proteins in clinical or research samples.

Basic Information

Product Name	(Lys(Ac)12)-Histone H4 (1-21)-Gly-Gly-Lys(Biotinyl)
CAS No.	2022956-66-5
Molecular Formula	C184H312N56O58S
Molecular Weight	~4250.9 g/mol (monoisotopic)
Synonyms	Acetylated Histone H4 (1-21) Peptide; H4K12ac Peptide; Biotinylated Ac-Histone H4 (1-21); Histone H4 (1-21), Nε-acetyl-L-lysine12, Nε-biotinyl-L-lysine21; Ac-H4(1-21)-biotin; AcH4K12 Biotin Peptide; Epigenetic Research Peptide; Chromatin Peptide Substrate
EINECS	Contact for details

Quality Control

Every batch of our synthetic peptides is produced under GMP-like conditions and undergoes comprehensive analytical characterization to ensure identity, purity, and consistency. Certificates of Analysis (COA) are provided, detailing results from HPLC, MS, and amino acid analysis. Our quality system is designed to meet the stringent requirements of research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation. Aliquot into single-use vials to minimize freeze-thaw cycles and exposure to air.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Retention time and mass consistent with reference
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Amino Acid Analysis	Within ± 10% of theoretical
Water Content (KF)	≤ 5.0%
Acetic Acid Content (HPLC)	≤ 10.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.