Description

Iodoacetamide-15N is a stable isotope-labeled derivative of iodoacetamide, where the nitrogen atom is enriched with the 15N isotope. This compound is a critical alkylating agent used to selectively modify cysteine residues in proteins and peptides, enabling advanced proteomics research and structural biology studies. It is essential for researchers and manufacturers in the fields of biomolecular mass spectrometry, protein sequencing, and quantitative proteomics who require precise, isotopically labeled reagents for accurate mass tagging and analysis.

Application

• Quantitative Proteomics: Used as a stable isotope-coded affinity tag (ICAT) reagent for the differential labeling and quantification of cysteine-containing peptides in complex biological samples.
• Protein Structure Analysis: Employed to alkylate cysteine thiol groups, preventing disulfide bond formation and aiding in protein characterization via techniques like X-ray crystallography and NMR.
• Biomolecular Mass Spectrometry: Serves as a key reagent for peptide and protein sample preparation, improving ionization efficiency and facilitating accurate mass determination.
• Enzyme Activity Studies: Acts as an irreversible inhibitor of cysteine proteases and other enzymes with active-site cysteine residues, useful in mechanistic biochemical research.
• Pharmaceutical Research: Utilized in the development and analysis of biopharmaceuticals, particularly for mapping disulfide bonds and confirming protein drug structure.
• Diagnostic Assay Development: Applied in the design of diagnostic kits and assays that rely on specific protein modification and detection.

Basic Information

Product Name	Iodoacetamide-15N
CAS No.	287476-20-4
Molecular Formula	C2H4INO
Molecular Weight	186.96 g/mol (for 15N labeled form)
Synonyms	2-Iodoacetamide-15N; Iodoacetamide (15N); IAA-15N; Acetamide, 2-iodo- (15N); 15N-Iodoacetamide; N-(Iodoacetyl)amine-15N; 2-Iodo-N-(15N)acetamide; IAM-15N
EINECS	Contact for details

Quality Control

Our Iodoacetamide-15N is produced under strict quality management systems to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment, chemical purity, and absence of critical impurities. We adhere to relevant industry standards for stable isotope-labeled compounds, ensuring reliable and reproducible performance in your experiments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0% (Chemical Purity)
Isotopic Enrichment (15N)	≥98 atom %
Water Content (KF)	≤0.5%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.