Description

3-Epi-24R 25-Dihydroxy Vitamin D3 is a biologically significant metabolite of Vitamin D3, specifically a dihydroxylated derivative with a unique stereochemical configuration at the 3-position. This compound matters as a crucial reference standard and research chemical for investigating the complex metabolic pathways, receptor binding, and biological activity profiles of Vitamin D analogs. It is primarily needed by pharmaceutical R&D teams, analytical laboratories, and academic researchers focused on endocrinology, bone metabolism, and the development of novel Vitamin D-based therapeutics.

Application

• Pharmaceutical Reference Standard: Used for the qualitative and quantitative analysis (HPLC, LC-MS) of Vitamin D metabolites in research and quality control.
• Biochemical Research: Serves as a critical tool for studying the enzymatic activity of CYP24A1 and other hydroxylases involved in Vitamin D catabolism.
• Metabolic Pathway Studies: Employed to elucidate the specific biological roles and potency of different Vitamin D3 epimers and dihydroxy derivatives.
• Drug Discovery & Development: Acts as a key intermediate or comparator in the synthesis and evaluation of new Vitamin D receptor modulators for treating osteoporosis, psoriasis, and hyperparathyroidism.
• Clinical Diagnostics: Potential use in calibrating assays for the precise measurement of complex Vitamin D metabolite panels in serum.

Basic Information

Item	Details
Product Name	3-Epi-24R 25-Dihydroxy Vitamin D3
CAS No.	272776-87-1
Molecular Formula	C₂₇H₄₄O₃
Molecular Weight	416.64 g/mol
Synonyms	24R,25-Dihydroxy-3-epi-vitamin D3; 3-Epi-24R,25-(OH)₂D₃; (24R)-3-Epi-24,25-Dihydroxycholecalciferol; 3-Epimer of 24R,25-Dihydroxyvitamin D3; 24R,25-Dihydroxy-3-epi-cholecalciferol; 3-Epi-24R,25-Dihydroxyvitamin D3; 24R,25-Dihydroxy-3-epicalciferol
EINECS	Contact for details

Quality Control

Our 3-Epi-24R 25-Dihydroxy Vitamin D3 is produced and handled under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the container to reach room temperature in a desiccator before opening to minimize moisture uptake. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.