Description

H-Arg-Arg-Amc . 3Hcl is a tripeptide derivative and a specialized biochemical reagent. This compound is a critical substrate and inhibitor used in enzymatic research, particularly for studying protease activity and specificity. It is essential for researchers and manufacturers in the fields of pharmaceutical development, proteomics, and biochemical diagnostics who require high-purity, well-characterized peptide substrates.

Application

• Protease Substrate: Used as a selective fluorogenic or colorimetric substrate for various serine and cysteine proteases in enzyme kinetics studies.
• Drug Discovery Research: Employed in high-throughput screening (HTS) assays to identify and characterize novel protease inhibitors for therapeutic targets.
• Biochemical Assay Development: Serves as a key component in developing diagnostic kits and assays for measuring specific protease activity in biological samples.
• Enzyme Mechanism Studies: Utilized to investigate the catalytic mechanism and substrate specificity of arginine-specific proteases like trypsin and furin.
• Cell Biology Research: Applied in studies of intracellular proteolytic pathways and signal transduction mechanisms involving peptide cleavage.
• Quality Control Reagent: Acts as a reference standard in the quality control of recombinant protease production and activity validation.

Basic Information

Product Name	H-Arg-Arg-Amc . 3Hcl
CAS No.	263843-55-6
Molecular Formula	C20H40Cl3N11O3
Molecular Weight	589.96 g/mol
Synonyms	H-Arg-Arg-AMC trihydrochloride; Arg-Arg-7-amido-4-methylcoumarin hydrochloride; Boc-Arg-Arg-AMC (common precursor name); L-Arginyl-L-arginine 7-amido-4-methylcoumarin trihydrochloride; R-R-AMC; RR-AMC; Trypsin substrate IV (AMC); Fluorogenic trypsin substrate
EINECS	Contact for details

Quality Control

Our H-Arg-Arg-Amc . 3Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, MS for identity confirmation, and NMR for structural verification. We provide Certificates of Analysis (COA) with detailed chromatograms and spectral data to ensure traceability and batch-to-batch consistency for your critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.