Description

Crotonaldehyde 2,4-Dinitrophenylhydrazone-D3 is a deuterium-labeled derivative of the classic 2,4-dinitrophenylhydrazone (2,4-DNPH) reagent, specifically designed for use as an internal standard in advanced analytical chemistry. This compound is critical for ensuring the accuracy and precision of quantitative analyses, particularly in mass spectrometry, by correcting for variations during sample preparation and instrument analysis. It is an essential tool for analytical laboratories, environmental testing facilities, and pharmaceutical R&D departments requiring high-fidelity measurement of carbonyl compounds like aldehydes and ketones.

Application

• Internal Standard for Mass Spectrometry (MS): Used as a stable isotope-labeled internal standard in LC-MS and GC-MS methods for the precise quantification of crotonaldehyde and other α,β-unsaturated aldehydes.
• Environmental Analysis: Enables accurate tracking and measurement of airborne carbonyl pollutants and volatile organic compounds (VOCs) in environmental monitoring and industrial hygiene studies.
• Pharmaceutical Impurity Profiling: Supports method development and validation for the detection and quantification of genotoxic impurities (GTIs) and degradation products in active pharmaceutical ingredients (APIs).
• Food Safety and Quality Control: Applied in the analysis of flavor compounds, spoilage markers, and process contaminants in food and beverage products.
• Metabolomics and Biomarker Research: Facilitates the accurate measurement of carbonyl-containing metabolites in biological fluids for disease biomarker discovery.
• Material Science Research: Used in studies analyzing degradation products and off-gassing from polymers, coatings, and other synthetic materials.

Basic Information

Product Name	Crotonaldehyde 2,4-Dinitrophenylhydrazone-D3
CAS No.	259824-64-1
Molecular Formula	C₁₀H₇D₃N₄O₄
Molecular Weight	241.22 g/mol
Synonyms	2,4-Dinitrophenylhydrazone of Crotonaldehyde-d3; (2E)-But-2-enal 2,4-dinitrophenylhydrazone-d3; Crotonaldehyde-DNPH-d3; Crotonaldehyde-d3 DNPH; Deuterated Crotonaldehyde 2,4-DNPH; 2,4-DNPH derivative of Crotonaldehyde-d3; Internal Standard for Crotonaldehyde Analysis-d3
EINECS	Contact for details

Quality Control

Every batch of our Crotonaldehyde 2,4-Dinitrophenylhydrazone-D3 is manufactured and controlled to meet the stringent requirements of analytical reference standard applications. We employ advanced chromatographic and spectroscopic techniques to ensure high chemical purity, isotopic enrichment, and batch-to-batch consistency. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for identity, purity (by HPLC), isotopic purity (D-content), and related substances. Our quality system supports compliance with relevant pharmacopeial and ISO guidelines for reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive (store away from light). For long-term storage, consider storing under inert atmosphere to maximize stability.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-content)	≥ 98 atom % D
Chemical Purity	≥ 95%
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.