Description

Fp-Biotin CAS NO 259270-28-5 is a high-purity, functionalized derivative of the essential vitamin biotin, designed for advanced biochemical conjugation and labeling applications. This compound is critical for researchers and manufacturers requiring precise, stable biotinylation of biomolecules for detection, purification, and diagnostic purposes. It is primarily utilized in the pharmaceutical, biotechnology, and life science research sectors for developing assays, targeted drug delivery systems, and diagnostic kits.

Application

• Conjugation reagent for the biotinylation of antibodies, proteins, and peptides in immunoassay development.
• Key intermediate in the synthesis of biotin-labeled probes for nucleic acid detection (e.g., FISH, microarrays).
• Preparation of affinity matrices and solid supports for the purification of biomolecules via streptavidin-biotin interaction.
• Component in the formulation of diagnostic kits and biosensors for clinical and research use.
• Tool compound in chemical biology for studying protein-protein interactions and cellular trafficking.
• Potential use in the development of antibody-drug conjugates (ADCs) and targeted therapeutics.
• Labeling agent for flow cytometry, immunohistochemistry (IHC), and ELISA applications.

Basic Information

Product Name	Fp-Biotin
CAS No.	259270-28-5
Molecular Formula	C19H32N4O5S
Molecular Weight	428.55 g/mol
Synonyms	Fluorescein-PEG-Biotin; 5-FAM-PEG4-Biotin; Fluorescein Biotin Conjugate; 5-Carboxyfluorescein-PEG4-Biotin; Biotin-PEG4-5-FAM; Biotinamidohexanoyl-6-aminohexanoic acid-5-fluorescein; Biotin-(PEG)4-5-FAM; Biotin, conjugate with fluorescein
EINECS	Contact for details

Quality Control

Our Fp-Biotin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional validation in conjugation assays. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Custom synthesis and purity grades (e.g., >95%, >98%) are available to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.