Description

Mca-Pro-β-Cyclohexyl-Ala-Gly-Nva-His-Ala-Dap(Dnp)-Nh2 is a synthetic, custom-designed peptide derivative featuring a dinitrophenyl (Dnp) labeled diaminopropionic acid (Dap) residue. This compound is of significant value in biochemical and pharmaceutical research, particularly as a specialized substrate, inhibitor, or labeled probe for studying enzyme kinetics and protein-protein interactions. It is primarily needed by research institutions, biotechnology companies, and pharmaceutical developers engaged in proteomics, assay development, and targeted therapeutic discovery.

Application

• Enzyme Substrate/Inhibitor Research: Used as a specific, chromogenic substrate for studying the activity and inhibition profiles of proteases and other hydrolytic enzymes due to the Dnp quenching group.
• Biochemical Assay Development: Serves as a key component in the development of high-throughput screening (HTS) assays and fluorescence resonance energy transfer (FRET)-based assays.
• Proteomics & Peptidomics: Employed as a reference standard or a modified peptide probe in mass spectrometry-based protein identification and characterization studies.
• Structure-Activity Relationship (SAR) Studies: Integral for investigating the impact of the β-cyclohexyl-alanine and norvaline (Nva) modifications on peptide stability, receptor binding, and biological activity.
• Pharmaceutical R&D: Acts as an intermediate or a lead compound in the discovery and optimization of novel peptide-based therapeutics targeting specific enzymes or receptors.

Basic Information

Product Name	Mca-Pro-β-Cyclohexyl-Ala-Gly-Nva-His-Ala-Dap(Dnp)-Nh2
CAS No.	256394-94-2
Molecular Formula	C48H64N14O14
Molecular Weight	1045.1 g/mol
Synonyms	7-Methoxycoumarin-4-acetyl-Pro-β-Cyclohexyl-Ala-Gly-Nva-His-Ala-Dap(Dnp)-NH2; Mca-Pro-Cha-Gly-Nva-His-Ala-Dap(Dnp)-NH2; FRET Peptide Substrate Mca-Pro-Cha-Gly-Nva-His-Ala-Dap(Dnp)-NH2; Dnp-Labeled Peptide; (7-Methoxycoumarin-4-yl)acetyl-Pro-cyclohexyl-Ala-Gly-Nva-His-Ala-L-2,3-diaminopropionyl(Dnp)-amide; Enzyme Substrate 256394-94-2; Custom Fluorescent Peptide
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Every batch of Mca-Pro-β-Cyclohexyl-Ala-Gly-Nva-His-Ala-Dap(Dnp)-Nh2 is characterized using advanced analytical techniques including HPLC, MS, and NMR to confirm identity, purity, and correct sequence. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (HPLC)	≥ 98%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 80%
Single Impurity (HPLC)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.