Description

3'-o-Moe-G(Ibu)-2'-Phosphoramidite is a specialized phosphoramidite monomer, a critical building block in the solid-phase synthesis of oligonucleotides. This compound is essential for the automated production of modified RNA strands, enabling the introduction of a 2'-O-methoxyethyl (MOE) sugar modification and an isobutyryl (Ibu) protecting group on the guanine base. It is primarily required by biotechnology and pharmaceutical companies engaged in the research and development of therapeutic nucleic acids, including antisense oligonucleotides (ASOs) and siRNA. The product is supplied under stringent quality control to ensure optimal coupling efficiency and purity for sensitive synthesis workflows.

Application

• Therapeutic Oligonucleotide Synthesis: A key raw material for the automated synthesis of antisense oligonucleotides (ASOs) and siRNA drug candidates.
• Research-Grade RNA Production: Used in academic and industrial R&D labs to synthesize modified RNA strands for target validation and mechanistic studies.
• High-Value Diagnostic Probe Manufacturing: Enables the production of nuclease-resistant, labeled RNA probes for advanced diagnostic assays.
• Synthesis of MOE-Modified Oligonucleotides: Specifically designed to incorporate the 2'-O-methoxyethyl (MOE) modification, which enhances binding affinity and metabolic stability.
• cGMP Manufacturing: Suitable for use in current Good Manufacturing Practice (cGMP) environments for the production of clinical trial materials.
• Scale-Up and Process Development: Supports process development and scale-up activities from milligram to kilogram scales for pre-clinical and clinical supply chains.

Basic Information

Item	Details
Product Name	3'-o-Moe-G(Ibu)-2'-Phosphoramidite
CAS No.	256224-06-3
Molecular Formula	C41H51N6O8P
Molecular Weight	786.87 g/mol
Synonyms	5'-O-Dimethoxytrityl-N2-isobutyryl-2'-O-methoxyethyl-guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-(2-methoxyethyl)-N2-(2-methyl-1-oxopropyl)-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 2'-MOE-G(Ibu)-CE Phosphoramidite; 2'-O-MOE-G(Ibu) Phosphoramidite; N2-Isobutyryl-2'-O-(2-methoxyethyl)-5'-O-(4,4'-dimethoxytrityl)guanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-(2-Methoxyethyl)guanosine phosphoramidite (Ibu protected); MOE-G(Ibu) Phosphoramidite
EINECS	Contact for details

Quality Control

Our 3'-o-Moe-G(Ibu)-2'-Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, NMR structural confirmation, and 31P NMR to verify phosphorous integrity. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided for every shipment. We support customer audits and can supply materials that align with cGMP guidelines for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store the product at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. Due to its hygroscopic and moisture-sensitive nature, the container must be allowed to equilibrate to room temperature before opening to prevent condensation. For long-term stability, keep the original sealed packaging intact until immediate use.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Assay (31P NMR)	≥ 97.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.