Description

2,3-Bis(3-Fluorophenyl)-5-Phenyltetrazolium Chloride is a specialized tetrazolium salt derivative, a critical reagent in biochemical and pharmaceutical research. Its primary value lies in its role as a redox indicator and a precursor for formazan dyes, which are essential for cell viability and cytotoxicity assays. This compound is vital for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise measurement of metabolic activity is required.

Application

• Cell Viability and Proliferation Assays: Used as a substrate in MTT and related assays to quantify mitochondrial activity in living cells.
• Cytotoxicity Screening: Essential for high-throughput screening of new drug candidates and chemical libraries for toxic effects.
• Biochemical Research: Serves as an electron acceptor in enzymatic studies to measure dehydrogenase activity.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical and research use.
• Material Science Research: Investigated for potential applications in developing novel sensors and electroactive materials.

Basic Information

Product Name	2,3-Bis(3-Fluorophenyl)-5-Phenyltetrazolium Chloride
CAS No.	240800-46-8
Molecular Formula	C25H15ClF2N4
Molecular Weight	444.86 g/mol
Synonyms	2,3-Bis(3-fluorophenyl)-5-phenyl-2H-tetrazolium chloride; 3-(3-Fluorophenyl)-2,5-diphenyltetrazolium chloride (isomer); Tetrazolium Red Chloride (related analog); TTC derivative; MTT analog; Fluorinated tetrazolium salt; Cell counting kit-8 (CCK-8) related compound; WST assay reagent precursor
EINECS	Contact for details

Quality Control

Every batch of 2,3-Bis(3-Fluorophenyl)-5-Phenyltetrazolium Chloride is manufactured under strict quality management protocols. We ensure high purity and batch-to-batch consistency through comprehensive analytical testing, including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment away from direct light exposure to maintain stability and potency.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.