Description

n-4-(1-Pyrene)butyroylglycylglycyl-L-phenylalanine is a synthetic, fluorescently-labeled peptide derivative designed for advanced biochemical research and diagnostic applications. This compound is engineered to act as a highly sensitive substrate or probe, leveraging the strong fluorescence of the pyrene moiety for detection and quantification. It is primarily utilized by researchers and developers in the fields of proteomics, enzyme kinetics, and fluorescence-based assay development, particularly for studying protease and peptidase activity. Its specific design makes it a critical tool for high-throughput screening and mechanistic studies in pharmaceutical and life science laboratories.

Application

• Protease/Peptidase Substrate: Used as a fluorogenic or fluorescent substrate to study the activity, kinetics, and inhibition of specific proteolytic enzymes.
• Biochemical Assay Development: Serves as a key component in developing sensitive fluorescence-based assays for high-throughput screening (HTS) in drug discovery.
• Cell Biology Research: Employed as a tracer or probe in cellular uptake studies and intracellular protease localization experiments.
• Diagnostic Reagent Manufacturing: Incorporated into the formulation of diagnostic kits for detecting specific enzyme levels related to disease states.
• Bioconjugation & Labeling: Acts as a fluorescent tag for peptides or proteins in bioconjugation chemistry and biomolecular labeling strategies.
• Academic & Industrial R&D: Essential for fundamental research in biochemistry and for applied research in pharmaceutical development.

Basic Information

Product Name	n-4-(1-Pyrene)butyroylglycylglycyl-L-phenylalanine
CAS No.	228414-53-7
Molecular Formula	C₃₅H₃₁N₃O₅
Molecular Weight	573.64 g/mol
Synonyms	Pyrenebutyroyl-Gly-Gly-Phe-OH; 4-(1-Pyrenyl)butyryl-Gly-Gly-L-phenylalanine; N-(4-(1-Pyrenyl)butyryl)glycylglycyl-L-phenylalanine; Pbf-Gly-Gly-Phe; Fluorescent Peptide Substrate Pyrene-Gly-Gly-Phe; L-Phenylalanine, N-[4-(1-pyrenyl)butanoyl]glycylglycyl-; Pyrene-labeled GGP peptide
EINECS	Contact for details

Quality Control

Our n-4-(1-Pyrene)butyroylglycylglycyl-L-phenylalanine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure identity, purity, and performance consistency. Standard quality control includes HPLC for purity assessment, mass spectrometry (MS) for identity confirmation, and NMR for structural verification. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.