Description

6-n-Biotinylaminohexyl Hydrogenphosphonate is a specialized biotinylated phosphonate derivative, a key building block in bioconjugation and affinity labeling. This compound is engineered to bridge biotin's high-affinity binding to streptavidin/avidin with the stable, metal-chelating properties of a phosphonate group. It is primarily utilized by researchers and manufacturers in the life sciences sector for developing advanced diagnostic assays, targeted drug delivery systems, and novel biomaterials.

Application

• Bioconjugation Reagent: For site-specific labeling of proteins, antibodies, and peptides with biotin for detection and purification.
• Affinity Chromatography Ligand Synthesis: Used to create novel stationary phases for the purification of metal-binding proteins or phosphonate-specific biomolecules.
• Diagnostic Assay Development: Serves as a critical component in ELISA, lateral flow assays, and other biosensors requiring a stable biotin-streptavidin interaction coupled with additional functional handles.
• Targeted Drug Delivery: Facilitates the development of biotin-drug conjugates where the phosphonate moiety can be used for bone targeting or as a linker for further chemical modification.
• Surface Functionalization: Enables the modification of nanoparticles, chips, and other surfaces for biomolecular immobilization studies.
• Chemical Biology Probes: Acts as a tool compound for studying enzyme inhibition, particularly those interacting with phosphate or phosphonate groups, via biotin-based pull-down assays.

Basic Information

Product Name	6-n-Biotinylaminohexyl Hydrogenphosphonate
CAS No.	224583-35-1
Molecular Formula	C16H32N4O5PS
Molecular Weight	423.50 g/mol
Synonyms	6-(Biotinylamino)hexyl Hydrogenphosphonate; Biotinyl-6-aminohexyl Hydrogenphosphonate; N-Biotinyl-1,6-diaminohexane Hydrogenphosphonate; 6-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexyl Hydrogenphosphonate; Biotin-aminocaproyl Hydrogenphosphonate; Biotin-PEG6-Phosphonate (informal); Biotinylated Alkylphosphonate
EINECS	Contact for details

Quality Control

Our 6-n-Biotinylaminohexyl Hydrogenphosphonate is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture. A detailed Certificate of Analysis (COA) is provided, ensuring traceability and compliance with research-grade material standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.