Description

(Z-Asp-Glu-Val-Asp)2-Rhodamine 110 is a high-purity, cell-permeable fluorogenic substrate designed for the sensitive detection of caspase-3 and caspase-7 activity. This compound matters because it provides a critical tool for researchers studying apoptosis (programmed cell death) in real-time, enabling accurate quantification of enzyme activity in live cells and cell lysates. It is essential for pharmaceutical R&D, academic research, and biotechnology companies focused on oncology, neurobiology, and drug discovery, where monitoring caspase activation is a key indicator of therapeutic efficacy and mechanism of action.

Application

• Apoptosis (Cell Death) Assays: Primary substrate for fluorometric and flow cytometry-based detection of caspase-3/7 activity in live cells.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and validate novel pro-apoptotic or anti-apoptotic compounds.
• Biomedical Research: Studying the role of caspases in cancer, neurodegenerative diseases (e.g., Alzheimer's, Parkinson's), and ischemic injury.
• Cell Biology Studies: Real-time imaging and kinetic analysis of caspase activation during various cellular stress responses.
• Diagnostic Development: A key component in developing clinical assays for monitoring treatment response in chemotherapy.
• Toxicology Testing: Assessing compound-induced cytotoxicity and organ damage in preclinical models.

Basic Information

Product Name	(Z-Asp-Glu-Val-Asp)2-Rhodamine 110
CAS No.	223538-61-2
Molecular Formula	C66H86N14O27
Molecular Weight	1451.47 g/mol
Synonyms	bis-(Z-DEVD)-R110; (Z-DEVD)2-Rhodamine 110; Caspase-3/7 Substrate (Z-DEVD)2-R110; Fluorogenic Caspase-3 Substrate; (Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe))2-Rhodamine 110; bis(Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe))-Rhodamine 110; (Z-DEVD)2-R110; AAT Bioquest Cat# 11002
EINECS	Contact for details

Quality Control

Our (Z-Asp-Glu-Val-Asp)2-Rhodamine 110 is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for life science research. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95% (Area Percent)
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffer
Functional Activity	Produces a dose-dependent increase in fluorescence (λex ~496 nm, λem ~520 nm) upon cleavage by recombinant caspase-3.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.