Description

6-Chloro-3-Indolyl-β-D-Gluconoride Sodium Salt is a specialized chromogenic substrate widely used in enzymatic detection systems. This compound matters for its high sensitivity and specificity in revealing the presence of β-glucuronidase activity, a key diagnostic enzyme marker. It is primarily needed by manufacturers and researchers in the life sciences sector for applications in microbiology, molecular biology, and diagnostic assay development.

Application

• Microbiological Media: As a key component in selective and differential culture media, such as MI Agar, for the detection and enumeration of E. coli and other coliform bacteria based on β-glucuronidase (GUD) activity.
• Diagnostic Reagents: Used in the formulation of diagnostic kits and reagents for clinical and food safety testing to identify specific bacterial strains.
• Molecular Biology Research: Employed as a substrate in histochemical and cytochemical staining procedures to localize GUS (β-glucuronidase) reporter gene expression in plant and animal tissues.
• Enzyme Assay Development: Serves as a critical reagent in developing and optimizing in vitro enzymatic assays for biochemical research and high-throughput screening.
• Water & Environmental Testing: Integral to testing protocols for the rapid detection of fecal contamination in water samples by targeting indicator organisms.
• Pharmaceutical Quality Control: Applied in sterility testing and microbial limit tests within pharmaceutical manufacturing environments.

Basic Information

Product Name	6-Chloro-3-Indolyl-β-D-Gluconoride Sodium Salt
CAS No.	216971-56-1
Molecular Formula	C20H24ClNNaO8
Molecular Weight	464.85 g/mol
Synonyms	6-Chloro-3-indolyl-β-D-glucuronide sodium salt; 6-Chloro-3-indolyl-β-D-glucopyranosiduronic acid sodium salt; 6-Cl-3-Indoxyl-β-D-Glucuronide Sodium Salt; 6-Chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt (related); X-GlucNa; Sodium 6-chloro-3-indolyl-β-D-glucuronide; 6-Chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid sodium salt; Magenta-Gluc
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indolyl-β-D-Gluconoride Sodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for critical diagnostic and research applications. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Off-white to pale yellow powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO; slightly soluble in methanol
Heavy Metals	≤ 20 ppm
Microbial Limits	Meets Ph. Eur. 2.6.12 / USP <61> requirements

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.