Description

6-((7-Amino-4-Methylcoumarin-3-Acetyl)Amino)Hexanoic Acid, Succinimidyl Ester is a highly reactive, amine-reactive fluorescent labeling reagent. This compound is critical for researchers and manufacturers who require precise, covalent attachment of a bright blue-fluorescent coumarin tag to proteins, peptides, antibodies, and other biomolecules containing primary amines. It is primarily needed by the life sciences, diagnostics, and pharmaceutical development sectors for applications in fluorescence microscopy, immunoassays, and protein tracking.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to proteins, antibodies, peptides, and oligonucleotides for detection and analysis.
• Proteomics & Bioimaging: Used as a tag in fluorescence microscopy (e.g., confocal, super-resolution) to visualize cellular structures and protein localization.
• Diagnostic Assay Development: Labeling of capture antibodies or antigens in fluorescence-based immunoassays (ELISA, Western Blot, flow cytometry).
• Drug Discovery & High-Throughput Screening (HTS): Tagging of target molecules to monitor binding events and enzymatic activity in assay platforms.
• Bioconjugation & Chemical Biology: A key building block for synthesizing advanced fluorescent probes and drug conjugates.
• Material Science: Functionalization of surfaces (e.g., nanoparticles, polymers) with fluorescent markers for sensing and tracking applications.

Basic Information

Product Name	6-((7-Amino-4-Methylcoumarin-3-Acetyl)Amino)Hexanoic Acid, Succinimidyl Ester
CAS No.	216309-02-3
Molecular Formula	C22H26N4O7
Molecular Weight	458.47 g/mol
Synonyms	AMCA-X, SE; 7-Amino-4-methylcoumarin-3-acetic acid, succinimidyl ester; AMCA succinimidyl ester; AMCA-OSu; 6-[(7-Amino-4-methyl-2-oxo-2H-chromen-3-yl)acetamido]hexanoic acid N-hydroxysuccinimide ester; N-Succinimidyl 6-((7-amino-4-methylcoumarin-3-acetyl)amino)hexanoate; AMCA-X, SE (AMCA-X succinimidyl ester); Fluorescent labeling reagent AMCA-X
EINECS	Contact for details

Quality Control

Our 6-((7-Amino-4-Methylcoumarin-3-Acetyl)Amino)Hexanoic Acid, Succinimidyl Ester is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, IR), to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.