Description

5-Acetamidofluorescein-Di-(B-D-Galactopyranoside) is a specialized fluorogenic substrate designed for the sensitive detection of β-galactosidase activity. This compound matters for researchers and diagnostic developers as it offers a high signal-to-noise ratio, enabling precise enzymatic assays and cellular imaging. It is primarily needed by the biotechnology, pharmaceutical, and diagnostic industries for applications in gene expression studies, cell-based reporter assays, and the development of diagnostic kits.

Application

• β-Galactosidase Reporter Gene Assays: A key substrate for quantifying gene expression in molecular biology and cell biology research.
• Cell-Based Screening: Used in high-throughput screening (HTS) platforms to identify modulators of gene expression or enzymatic activity.
• Histochemistry & Microscopy: Enables the visualization of β-galactosidase expression in tissues and fixed cells for developmental biology and pathology studies.
• Diagnostic Kit Development: Serves as a critical component in the formulation of enzymatic diagnostic tests for clinical and research laboratories.
• Enzyme Kinetics Studies: Employed to study the catalytic mechanism and inhibition of β-galactosidase enzymes.
• Flow Cytometry Applications: Useful for detecting and sorting cells based on β-galactosidase reporter activity.

Basic Information

Product Name	5-Acetamidofluorescein-Di-(B-D-Galactopyranoside)
CAS No.	216299-45-5
Molecular Formula	C35H35NO18
Molecular Weight	757.65 g/mol
Synonyms	5-Acetamidofluorescein Di-β-D-Galactopyranoside; FDG (5-Acetamidofluorescein Derivative); 5-Acetamidofluorescein Digalactoside; β-Galactosidase Substrate, Fluorogenic; C12FDG; 5-Acetamidofluorescein Di-(β-D-Galactoside); Fluorescein Di-β-D-Galactopyranoside, 5-Acetamido Derivative
EINECS	Contact for details

Quality Control

Our 5-Acetamidofluorescein-Di-(B-D-Galactopyranoside) is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is characterized by advanced analytical techniques, including HPLC and NMR, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF
Fluorogenic Activity	Functional test passed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.