Description

4-Chloromethyl-6,8-Difluoroumbelliferyl-β-D-Galactopyranoside is a specialized fluorogenic substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of β-galactosidase activity. It is primarily utilized in molecular biology, diagnostic kit development, and pharmaceutical research where reliable and specific enzyme detection is paramount.

Application

• Fluorogenic Substrate for β-Galactosidase: Used as a key reagent in enzymatic assays to detect and quantify β-galactosidase activity with high sensitivity via fluorescence.
• Molecular Biology Research: Essential for reporter gene assays (e.g., lacZ gene expression), cell signaling studies, and genetic screening applications.
• Diagnostic Development: Incorporated into the formulation of diagnostic kits and test strips for clinical and point-of-care testing.
• Pharmaceutical & Drug Discovery: Employed in high-throughput screening (HTS) platforms to identify enzyme inhibitors or activators for therapeutic development.
• Microbiology & Cell Biology: Used to study bacterial gene expression (E. coli lac operon) and for staining applications in histochemistry and flow cytometry.
• Biochemical Reagent Manufacturing: Serves as a critical raw material for producers of research-grade biochemicals and assay kits.

Basic Information

Product Name	4-Chloromethyl-6,8-Difluoroumbelliferyl-β-D-Galactopyranoside
CAS No.	215868-46-5
Molecular Formula	C20H16ClF2NO8
Molecular Weight	471.79 g/mol
Synonyms	6,8-Difluoro-4-(chloromethyl)umbelliferyl β-D-galactopyranoside; FDG (Fluorescein Di-β-D-Galactopyranoside) analog; Chloromethyl-DFUG; CMFDUG; β-Galactosidase Fluorogenic Substrate; 4-Chloromethyl-6,8-difluoro-7-hydroxycoumarin-β-D-galactopyranoside; CM-6,8-Difluoroumbelliferyl-β-D-Galactoside
EINECS	Contact for details

Quality Control

Our production of 4-Chloromethyl-6,8-Difluoroumbelliferyl-β-D-Galactopyranoside adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, desiccate and keep frozen. Allow the product to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Enzymatic Activity	Suitable for use as a fluorogenic substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.