Description

Pacific Blue Succinimidyl Ester is a reactive, amine-reactive fluorescent dye derivative widely used for labeling biomolecules. This compound is critical for creating highly sensitive and stable fluorescent conjugates for advanced detection and imaging applications. It is essential for researchers and manufacturers in the fields of flow cytometry, fluorescence microscopy, immunoassay development, and nucleic acid analysis who require bright, photostable blue-fluorescent probes.

Application

• Antibody and Protein Labeling: Conjugation to primary amines for use in fluorescence-activated cell sorting (FACS), western blotting, and immunohistochemistry.
• Oligonucleotide and Nucleic Acid Probes: Labeling of amino-modified DNA or RNA for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Biomolecular Interaction Studies: Tagging of peptides, proteins, or other ligands for fluorescence polarization (FP) and Förster resonance energy transfer (FRET) assays.
• Diagnostic Kit Manufacturing: Production of fluorescent detection reagents for clinical and research immunoassays.
• Live-Cell Imaging: Preparation of fluorescent tracers for tracking cellular processes, subject to validation of cell permeability and toxicity.
• Material Science & Bioconjugation: Functionalization of surfaces, nanoparticles, or polymers with fluorescent markers for sensor development.

Basic Information

Product Name	Pacific Blue Succinimidyl Ester
CAS No.	215868-33-0
Molecular Formula	C25H21N3O7S2
Molecular Weight	539.58 g/mol
Synonyms	Pacific Blue™ NHS Ester; PBX NHS Ester; 6,8-Difluoro-7-hydroxy-4-methyl-2-oxo-2H-1-benzopyran-3-carboxylic Acid Succinimidyl Ester; 6,8-Difluoro-7-hydroxy-4-methylcoumarin-3-carboxylic Acid N-Succinimidyl Ester; Sulfo-Cyanine5 NHS Ester (Note: This is a common mis-association, but included for search clarity); Fluorescent NHS Ester Dye; Amine-reactive blue fluorescent dye
EINECS	Contact for details

Quality Control

Our Pacific Blue Succinimidyl Ester is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Blue to dark blue solid or film
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	~410 - 420 nm
Emission (λmax)	~450 - 460 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.