Description

Succinimidyl-2-(Iodoacetamido)Acetate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a sulfhydryl-reactive iodoacetyl group. This compound is critical for creating stable, covalent linkages between proteins, peptides, and other biomolecules, enabling advanced bioconjugation strategies. It is an essential tool for researchers and manufacturers in biopharmaceutical development, diagnostic assay production, and proteomics research, where precise and irreversible conjugation is required.

Application

• Antibody-Drug Conjugate (ADC) Linker Chemistry: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein-Protein Crosslinking: To study protein interactions, create protein complexes, or stabilize protein structures for analysis.
• Peptide & Protein Labeling: For attaching fluorescent dyes, biotin, or other probes to cysteine-containing biomolecules for detection and imaging.
• Immobilization of Biomolecules: For covalently attaching proteins or peptides to solid supports (e.g., sensor chips, beads, plates) to create bioactive surfaces.
• Diagnostic Reagent Manufacturing: In the production of enzyme conjugates and labeled antibodies used in ELISA, lateral flow assays, and other immunoassays.
• Polymer Functionalization: To introduce reactive handles onto polymers or nanoparticles for subsequent bioconjugation.

Basic Information

Product Name	Succinimidyl-2-(Iodoacetamido)Acetate
CAS No.	215099-66-4
Molecular Formula	C9H10IN2O6
Molecular Weight	378.09 g/mol
Synonyms	N-Succinimidyl Iodoacetate; SIA; NHS-Iodoacetate; 2,5-Dioxopyrrolidin-1-yl 2-(2-iodoacetamido)acetate; N-(Iodoacetyloxy)succinimide; Iodoacetyl NHS Ester; Succinimidyl iodoacetate; SIAC
EINECS	Contact for details

Quality Control

Our Succinimidyl-2-(Iodoacetamido)Acetate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and bioconjugation applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to cGMP principles where applicable to support our customers in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.