Description

Hydroxyurea-15N CAS NO 214331-53-0 is a stable isotope-labeled analog of the antineoplastic agent hydroxyurea, where the nitrogen atom is enriched with the 15N isotope. This labeled compound is an essential tool in pharmaceutical research and development, enabling precise pharmacokinetic, metabolic, and mechanistic studies through advanced analytical techniques like mass spectrometry and NMR. It is primarily utilized by research institutions, pharmaceutical companies, and contract research organizations (CROs) engaged in oncology drug development and metabolic pathway analysis.

Application

• Internal Standard for Quantitative Analysis: Serves as a critical internal standard in LC-MS and GC-MS methods for the accurate quantification of unlabeled hydroxyurea in biological matrices, ensuring reliable pharmacokinetic data.
• Metabolic Pathway Tracing: Used in tracer studies to elucidate the metabolic fate and biotransformation pathways of hydroxyurea, providing insights into its mechanism of action and potential metabolites.
• Drug-Drug Interaction Studies: Facilitates research into how hydroxyurea interacts with other medications, helping to assess potential synergistic or inhibitory effects.
• Biomarker Research: Aids in the development and validation of assays for hydroxyurea as a potential biomarker in clinical settings.
• Stable Isotope-Labeled Reference Material: Employed as a certified reference material (CRM) to calibrate analytical instruments and validate method accuracy in regulatory-compliant laboratories.
• Chemical Mechanism Investigation: Used in fundamental chemical research to study reaction mechanisms involving hydroxyurea through isotope effects observed in 15N-NMR spectroscopy.

Basic Information

Product Name	Hydroxyurea-15N
CAS No.	214331-53-0
Molecular Formula	C15NH4O2
Molecular Weight	77.05 g/mol (for 15N labeled form)
Synonyms	Hydroxycarbamide-15N; 15N-Hydroxyurea; Carbamohydroxamic acid-15N; N-Hydroxycarbamide-15N; Litalir-15N; Hydrea-15N; Urea, hydroxy-15N; SQ-1089-15N; NSC-32065-15N
EINECS	Contact for details

Quality Control

Every batch of Hydroxyurea-15N is manufactured and analyzed under strict quality management systems. We provide comprehensive analytical data to confirm chemical purity, isotopic enrichment (typically ≥98 atom % 15N), and the absence of critical impurities. A detailed Certificate of Analysis (COA) is supplied with each shipment, documenting results from tests including HPLC, NMR, and mass spectrometry. Our quality commitment ensures the material is suitable for demanding research and regulatory submission support.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥98 atom % 15N
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.