Description

Ac-Leu-Glu-Thr-Asp-Afc is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound matters for researchers and quality control labs requiring precise detection and quantification of specific protease activity. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, biochemical research, and assay development.

Application

• Protease Activity Assays: Used as a selective fluorogenic substrate for monitoring the enzymatic activity of caspases and other related proteases in apoptosis research.
• High-Throughput Screening (HTS): Enables rapid, sensitive detection in drug discovery platforms to identify potential enzyme inhibitors or activators.
• Biochemical Research: Serves as a critical tool for studying enzyme kinetics, mechanism of action, and substrate specificity in purified enzyme systems.
• Diagnostic Development: Employed in the development of clinical diagnostic kits for diseases where specific protease levels are a biomarker.
• Cell Biology Studies: Facilitates the real-time measurement of protease activity within live cells or cell lysates.
• Quality Control Testing: Applied in biopharmaceutical manufacturing to test the purity and activity of enzyme-based therapeutics.

Basic Information

Product Name	Ac-Leu-Glu-Thr-Asp-Afc
CAS No.	210345-02-1
Molecular Formula	C30H41N5O12
Molecular Weight	663.68 g/mol
Synonyms	Ac-LETD-AFC; Ac-Leu-Glu-Thr-Asp-7-Amino-4-trifluoromethylcoumarin; N-Acetyl-L-leucyl-L-α-glutamyl-L-threonyl-L-α-aspartyl-7-amino-4-trifluoromethylcoumarin; Caspase-8 Substrate, Ac-LETD-AFC; LETD-AFC; Fluorogenic Caspase-8 Substrate; Ac-LETD-AFC, Caspase 8 Substrate; AFC Caspase Substrate
EINECS	Contact for details

Quality Control

Our Ac-Leu-Glu-Thr-Asp-Afc is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) with detailed specifications are provided to guarantee the product meets the rigorous standards required for pharmaceutical and life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.