Description

Interleukin-4 CAS NO 207137-56-2 is a pleiotropic cytokine, specifically a type II interleukin, that plays a critical role in regulating immune responses, particularly in the differentiation of naive helper T cells to Th2 cells. This protein is essential for research and development in immunology, offering significant value for studying allergic inflammation, asthma, and antibody class switching. It is primarily needed by biotechnology and pharmaceutical companies, academic research institutions, and diagnostic developers focused on advancing therapeutic and diagnostic solutions for immune-mediated diseases.

Application

• Biopharmaceutical R&D: Used as a key reagent in the research and development of immunotherapies, vaccines, and treatments for autoimmune disorders and allergies.
• Cell Culture & Differentiation Studies: Essential for in vitro studies to induce and analyze the differentiation of B-cells and T-cells, particularly towards a Th2 phenotype.
• Diagnostic Assay Development: Serves as a standard or critical component in the development of immunoassays (ELISA, Luminex) to detect and quantify immune responses.
• Signal Transduction Research: Utilized to investigate the JAK-STAT signaling pathway and understand mechanisms of cytokine receptor activation and downstream effects.
• Preclinical Model Studies: Applied in animal and cellular models to study the pathophysiology of diseases like asthma, atopic dermatitis, and helminth infections.
• Quality Control & Standardization: Acts as a reference standard for the quality control and potency testing of related biological products and cell-based assays.

Basic Information

Product Name	Interleukin-4
CAS No.	207137-56-2
Molecular Formula	C600H952N168O184S8 (Approx., for human IL-4)
Molecular Weight	~15-18 kDa (Glycosylated form dependent)
Synonyms	IL-4; B-Cell Stimulatory Factor-1 (BSF-1); B-Cell Growth Factor-1 (BCGF-1); Lymphocyte Stimulatory Factor-1; Mast Cell Growth Factor-2 (MCGF-2); T-Cell Growth Factor-2 (TCGF-2); Human Interleukin-4; Recombinant Human IL-4
EINECS	Contact for details

Quality Control

Our Interleukin-4 is produced under stringent conditions and undergoes rigorous quality testing to ensure high purity, biological activity, and consistency. Each batch is characterized using a battery of analytical techniques including SDS-PAGE, HPLC, and mass spectrometry. Biological activity is confirmed by cell proliferation assays. We provide comprehensive Certificates of Analysis (COA) detailing purity, endotoxin levels, sterility (where applicable), and specific activity, supporting compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, it is recommended to keep the lyophilized product at -20°C or below. Reconstituted solutions should be aliquoted and stored at -20°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed properly after each use in a dry environment.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (HPLC/MS)	Conforms to standard
Purity (SDS-PAGE & HPLC)	≥ 95%
Endotoxin Level (LAL)	< 1.0 EU/μg
Biological Activity (Cell-based assay)	≥ 1 x 106 units/mg
Moisture Content (KF)	≤ 3.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.