Description

L-Valine-13C5, 15N is a high-purity, stable isotope-labeled analog of the essential amino acid L-Valine, uniformly labeled with carbon-13 and nitrogen-15. This compound is a critical tool for advanced metabolic research, providing unparalleled accuracy in mass spectrometry-based quantification and tracer studies. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and CROs specializing in proteomics, metabolomics, and pharmacokinetic studies.

Application

• Quantitative Proteomics & Mass Spectrometry: Serves as an internal standard for the precise absolute quantification of proteins and peptides using techniques like SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and AQUA.
• Metabolic Flux Analysis (MFA): Used as a tracer to map and quantify intracellular metabolic pathways in cell cultures, tissues, or whole organisms.
• Drug Metabolism & Pharmacokinetics (DMPK): Enables accurate tracking of drug candidates and their metabolites in biological systems for ADME studies.
• NMR Spectroscopy: Facilitates structural and dynamic studies of biomolecules by providing non-radioactive, NMR-active isotopes.
• Clinical Research & Diagnostics: Supports the development of advanced diagnostic assays for metabolic disorders by providing a reference standard.
• Nutritional Science Research: Investigates amino acid metabolism, protein synthesis, and turnover in nutritional studies.

Basic Information

Product Name	L-Valine-13C5, 15N
CAS No.	202407-30-5
Molecular Formula	13C5H1115NO2
Molecular Weight	124.10 (based on isotopic composition)
Synonyms	(2S)-2-Amino-3-methylbutanoic-13C5,15N; L-Valine (13C5, 15N); Valine-13C5,15N; (S)-2-Azanyl-3-methyl-13C5,15N-butanoic acid; Stable Isotope Labeled L-Valine; U-13C5,15N-L-Valine; SILAC Amino Acid 13C5,15N-Valine; AQUA Peptide Standard Grade Valine
EINECS	Contact for details

Quality Control

Every batch of L-Valine-13C5, 15N is manufactured and tested under strict quality systems to ensure the highest isotopic and chemical purity for sensitive research applications. Our products undergo rigorous quality testing, including advanced spectroscopic and chromatographic methods, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing isotopic enrichment (typically >98-99 atom % 13C and 15N), chemical purity, and specific analytical data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This material is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere after each use to prevent moisture uptake and degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 98 atom % 13C ≥ 98 atom % 15N
Optical Rotation	[α]20/D: +26° to +30° (c=1 in H2O)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.