Description

L-Proline-13C5,15N is a stable isotope-labeled analog of the amino acid L-proline, where all five carbon atoms are the 13C isotope and the nitrogen atom is the 15N isotope. This compound is essential for advanced quantitative proteomics, metabolic flux analysis, and pharmaceutical research requiring precise internal standards for mass spectrometry. It is a critical reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and academic sectors who require high-purity, isotopically enriched building blocks for tracer studies and analytical method development.

Application

• Mass Spectrometry Internal Standard: Used as a quantitative reference standard in LC-MS/MS and GC-MS for accurate measurement of proline and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing (Fluxomics): Enables the detailed study of metabolic pathways, including collagen biosynthesis, plant stress responses, and microbial fermentation processes.
• Pharmaceutical Drug Development: Serves as a labeled precursor in the synthesis of isotopically labeled drug candidates for pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies.
• Nutritional & Clinical Research: Employed in human and animal studies to investigate protein metabolism, wound healing, and connective tissue disorders.
• Biomarker Discovery & Validation: Facilitates the accurate identification and quantification of proline-containing peptides and biomarkers in disease state profiling.
• NMR Spectroscopy: Provides a non-radioactive tracer for structural and dynamic studies of proteins and peptides using nuclear magnetic resonance.

Basic Information

Item	Detail
Product Name	L-Proline-13C5,15N
CAS No.	202407-23-6
Molecular Formula	¹³C₅H₉¹⁵NO₂
Molecular Weight	120.09 (based on ¹³C=13.003355, ¹⁵N=15.000109)
Synonyms	(2S)-Pyrrolidine-2-carboxylic acid-13C5,15N; L-(-)-Proline-13C5,15N; Proline-13C5,15N; (S)-Proline-13C5,15N; 13C5,15N-L-Proline; Isotopically labeled L-proline; 13C5 15N Proline; Stable isotope labeled proline
EINECS	Contact for details

Quality Control

Every batch of L-Proline-13C5,15N is manufactured and tested under strict quality management systems. We guarantee high chemical and isotopic purity to meet the exacting demands of analytical and synthetic applications. Comprehensive testing includes isotopic enrichment (by NMR or MS), chiral purity, chemical purity (by HPLC), and identity confirmation. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % ¹³C; ≥ 98 atom % ¹⁵N
Optical Rotation	-83° to -87° (c=1 in H₂O)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.