Description

Cytidine-1'-13C is a stable isotope-labeled nucleoside analog where the carbon-1' position of the ribose sugar is enriched with the 13C isotope. This precise labeling is critical for advanced research requiring high specificity in metabolic and pharmacokinetic studies. It is an essential biochemical tool for scientists in pharmaceutical development, academic research, and diagnostic manufacturing who require isotopically labeled standards for mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy.

Application

• Internal Standard for Quantitative Analysis: Used as a certified reference material (CRM) in LC-MS/MS and GC-MS for the accurate quantification of cytidine and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Enables detailed investigation of nucleoside and nucleotide metabolism, RNA synthesis, and salvage pathways in cell culture and in vivo studies.
• Pharmaceutical Drug Development: Serves as a key tracer in Absorption, Distribution, Metabolism, and Excretion (ADME) studies for novel antiviral and anticancer nucleoside analogs.
• NMR Spectroscopy Studies: Provides a non-radioactive probe for elucidating molecular structure, dynamics, and binding interactions in biochemical systems.
• Diagnostic Assay Development: Employed in the calibration and validation of clinical diagnostic kits that measure nucleoside levels as disease biomarkers.
• Biochemical Research Reagent: Used in enzymatic studies and as a precursor for the synthesis of labeled nucleotides (e.g., CTP-1'-13C).

Basic Information

Item	Details
Product Name	Cytidine-1'-13C
CAS No.	201996-57-8
Molecular Formula	C9H1313CN3O5
Molecular Weight	Contact for details
Synonyms	1'-13C-Cytidine; Cytidine (1'-13C); 4-Amino-1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)-1-13C)tetrahydrofuran-2-yl)pyrimidin-2(1H)-one; 1-(β-D-Ribofuranosyl)-1-13C-cytosine; [1'-13C]Cytidine; Labeled Cytidine; Isotopic Cytidine; Stable Isotope-Labeled Nucleoside
EINECS	Contact for details

Quality Control

Every batch of Cytidine-1'-13C is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing via NMR, MS, and HPLC to ensure identity, potency, and purity meet exacting research standards. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific data for isotopic enrichment, chemical purity, and solvent residues.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.