Description

Z-Gly-Pro-Arg-Amc Hcl is a fluorogenic peptide substrate specifically designed for the sensitive detection and quantification of thrombin and related serine protease activity. This compound matters for researchers and manufacturers requiring precise, high-throughput enzymatic assays in diagnostic and pharmaceutical development. It is primarily needed by the life sciences, diagnostic reagent, and pharmaceutical research industries for applications in hemostasis research, drug discovery, and clinical chemistry.

Application

• Fluorogenic Substrate for Thrombin: Used in continuous kinetic assays to measure thrombin activity with high sensitivity and specificity.
• Diagnostic Reagent Manufacturing: A key component in the production of test kits for coagulation disorders and thrombotic risk assessment.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel anticoagulant or procoagulant compounds.
• Biochemical Research: Essential for studying the kinetics, inhibition, and physiological roles of thrombin and other trypsin-like proteases.
• Quality Control in Biologics: Used to monitor and validate the activity of thrombin preparations used in therapeutic products or surgical procedures.

Basic Information

Product Name	Z-Gly-Pro-Arg-Amc Hcl
CAS No.	201928-42-9
Molecular Formula	C28H41N9O7 • xHCl
Molecular Weight	Contact for details
Synonyms	Benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin Hydrochloride; Z-Gly-Pro-Arg-AMC • HCl; Z-GPR-AMC Hydrochloride; Thrombin Substrate I (fluorogenic); Boc-Gly-Pro-Arg-AMC (common alias); 7-Amido-4-methylcoumarin derivative of Z-Gly-Pro-Arg; Fluorogenic thrombin peptide substrate.
EINECS	Contact for details

Quality Control

Our Z-Gly-Pro-Arg-Amc Hcl is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference standard in thrombin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.