Description

H-Lys-Ala-Amc Hcl is a synthetic peptide derivative, specifically a fluorogenic substrate containing the 7-amino-4-methylcoumarin (AMC) leaving group. This compound is a critical reagent in enzymatic research and assay development, designed for high-sensitivity detection of protease activity. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on biochemistry and molecular biology.

Application

• Protease Activity Assays: As a fluorogenic substrate for the detection and quantification of specific proteolytic enzymes, including trypsin-like serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Enzyme Kinetic Studies: Employed to determine kinetic parameters (Km, Vmax, kcat) of proteases, aiding in mechanistic biochemical research.
• Diagnostic Development: Serves as a key component in the development of sensitive fluorometric diagnostic tests for diseases involving dysregulated protease activity.
• Biochemical Research: A tool for studying protease function, specificity, and regulation in various biological pathways and disease models.
• Quality Control in Biologics: Can be used in purity and activity testing of enzyme-based biopharmaceutical products.

Basic Information

Product Name	H-Lys-Ala-Amc Hcl
CAS No.	201853-24-9
Molecular Formula	C19H27N5O4 • HCl
Molecular Weight	425.91 g/mol (free base: 389.45 g/mol)
Synonyms	L-Lysyl-L-alanine 7-amido-4-methylcoumarin hydrochloride; Lys-Ala-AMC HCl; Nα-(7-Amino-4-methylcoumarin-3-acetyl)-L-lysyl-L-alanine hydrochloride; H-Lys-Ala-AMC•HCl; Boc-Lys-Ala-AMC (deprotected form reference); Fluorogenic substrate for proteases; AMC-labeled peptide substrate; Protease substrate 201853-24-9.
EINECS	Contact for details

Quality Control

Our H-Lys-Ala-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and NMR for structural verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. For specific purity requirements (e.g., >95%, >98%), please contact our technical team.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.